全基因组关联研究揭示与甘蔗产量相关性状相关的数量性状基因座和基因。

Genome-Wide Association Study Unravels Quantitative Trait Loci and Genes Associated with Yield-Related Traits in Sugarcane.

机构信息

Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China.

State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, China.

出版信息

J Agric Food Chem. 2023 Nov 8;71(44):16815-16826. doi: 10.1021/acs.jafc.3c02935. Epub 2023 Oct 19.

DOI:10.1021/acs.jafc.3c02935

PMID:37856846

Abstract

Sugarcane, a major sugar and energy crop worldwide faces an increasing demand for higher yields. Identifying yield-related markers and candidate genes is valuable for breeding high-yield varieties using molecular techniques. In this work, seven yield-related traits were evaluated in a diversity panel of 159 genotypes, derived from , , and modern sugarcane genotypes. All traits exhibited significant genetic variance with high heritability and high correlations. Genetic diversity analysis reveals a genomic decay of 23 kb and an average single nucleotide polymorphism (SNP) number of 25,429 per genotype. These 159 genotypes were divided into 4 subgroups. Genome-wide association analysis identified 47 SNPs associated with brix, spanning 36 quantitative trait loci (QTLs), and 138 SNPs for other traits across 104 QTLs, covering all 32 chromosomes. Interestingly, 12 stable QTLs associated with yield-related traits were identified, which contained 35 candidate genes. This work provides markers and candidate genes for marker-assisted breeding to improve sugarcane yields.

摘要

甘蔗是一种全球主要的糖料和能源作物，面临着提高产量的巨大需求。鉴定与产量相关的标记和候选基因对于利用分子技术培育高产品种具有重要意义。在这项工作中，对来自、和现代甘蔗基因型的 159 个基因型的多样性群体进行了 7 个与产量相关的性状评估。所有性状均表现出显著的遗传方差，具有高遗传力和高相关性。遗传多样性分析显示，基因组衰减了 23 kb，每个基因型的平均单核苷酸多态性（SNP）数量为 25,429。这 159 个基因型被分为 4 个亚组。全基因组关联分析鉴定出 47 个与糖度相关的 SNP，跨越 36 个数量性状位点（QTL），以及 138 个与其他性状相关的 SNP，跨越 104 个 QTL，涵盖所有 32 条染色体。有趣的是，鉴定出 12 个与产量相关性状相关的稳定 QTL，其中包含 35 个候选基因。这项工作为提高甘蔗产量的标记辅助育种提供了标记和候选基因。