Guangxi Key Laboratory for Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, 530005, China.
Agronomy Department, IFAS, University of Florida, Gainesville, FL, 32611, USA.
Sci Rep. 2024 Jul 13;14(1):16214. doi: 10.1038/s41598-024-67059-w.
Leaf scald, caused by Xanthomonas albilineans, is a severe disease affecting sugarcane worldwide. One of the most practical ways to control it is by developing resistant sugarcane cultivars. It is essential to identify genes associated with the response to leaf scald. A panel of 170 sugarcane genotypes was evaluated for resistance to leaf scald in field conditions for 2 years, followed by a 1-year greenhouse experiment. The phenotypic evaluation data showed a wide continuous distribution, with heritability values ranging from 0.58 to 0.84. Thirteen single nucleotide polymorphisms (SNPs) were identified, significantly associated with leaf scald resistance. Among these, eight were stable across multiple environments and association models. The candidate genes identified and validated based on RNA-seq and qRT-PCR included two genes that encode NB-ARC leucine-rich repeat (LRR)-containing domain disease-resistance protein. These findings provide a basis for developing marker-assisted selection strategies in sugarcane breeding programs.
叶枯病由黄单胞菌引起，是一种严重影响全球甘蔗的疾病。防治该病最实用的方法之一是培育抗叶枯病的甘蔗品种。鉴定与叶枯病反应相关的基因至关重要。在田间条件下，对 170 个甘蔗基因型进行了 2 年的抗叶枯病评估，随后进行了 1 年的温室试验。表型评估数据显示出广泛的连续分布，遗传力值在 0.58 到 0.84 之间。鉴定出 13 个与抗叶枯病显著相关的单核苷酸多态性（SNP）。其中，8 个 SNP 在多个环境和关联模型中稳定存在。基于 RNA-seq 和 qRT-PCR 鉴定和验证的候选基因包括两个编码 NB-ARC 富含亮氨酸重复（LRR）的含域疾病抗性蛋白的基因。这些发现为甘蔗育种计划中的标记辅助选择策略的制定提供了依据。
