Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
Curr Opin Chem Biol. 2023 Dec;77:102403. doi: 10.1016/j.cbpa.2023.102403. Epub 2023 Oct 17.
Within their native milieu of the cell, the activities of enzymes are controlled by a range of factors including protein interactions and post-translational modifications. The involvement of these factors in fundamental cell biology and the etiology of diseases is stimulating interest in monitoring enzyme activities within tissues. The creation of synthetic substrates, and their use with different imaging modalities, to detect and quantify enzyme activities has great potential to propel these areas of research. Here we describe the latest developments relating to the creation of substrates for imaging and quantifying the activities of glycoside hydrolases, focusing on mammalian systems. The limitations of current tools and the difficulties within the field are summarised, as are prospects for overcoming these challenges.
在细胞的天然环境中，酶的活性受到一系列因素的控制，包括蛋白质相互作用和翻译后修饰。这些因素在细胞生物学基础和疾病病因学中的参与，激发了人们对监测组织内酶活性的兴趣。合成底物的创建及其与不同成像模式的结合，用于检测和量化酶活性，具有极大的推动这些研究领域的潜力。在这里，我们描述了与创建用于成像和定量测定糖苷水解酶活性的底物有关的最新进展，重点是哺乳动物系统。总结了当前工具的局限性和该领域的困难，并提出了克服这些挑战的前景。
