Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Department of Chemistry, Shanghai Stomatological Hospital, State Key Laboratory of Molecular Engineering of Polymers and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China.
Biosens Bioelectron. 2024 Jan 1;243:115759. doi: 10.1016/j.bios.2023.115759. Epub 2023 Oct 13.
Chiral molecules are abundant in nature. Phenylketonuria (PKU) is caused by the abnormal transformation of chiral molecules L-phenylalanine (L-Phe) in the human blood, which can cause irreversible harm to the human body. In this work, we documented an electrochemiluminescent (ECL) dual-mode sensor platform based on molecularly imprinted polymer (MIP) modified closed bipolar electrodes for high sensitivity detection of L-Phe and D-phenylalanine (D-Phe). In the anode chamber of a bipolar electrode modified with phenylalanine imprinting, Ru (bpy) underwent a redox reaction to produce a chemiluminescence response under the stimulation of a driving voltage. At the same time, the reduction of the cathode film of the bipolar electrode was promoted, and the color changed from dark blue to nearly white. Thus, the dual-mode detection of target molecules is realized. The detection range of the sensor for phenylalanine reached 0.01-10,000 nM, and the detection limits of L-Phe and D-Phe were 3.9 pM and 4.6 pM (S/N = 3), respectively. This dual-mode system achieved high stability and high specificity, and also successfully realized the detection of actual samples, which is expected to achieve future clinical applications.
手性分子在自然界中大量存在。苯丙酮尿症（PKU）是由人体血液中手性分子 L-苯丙氨酸（L-Phe）的异常转化引起的，会对人体造成不可逆转的伤害。在这项工作中，我们记录了一种基于分子印迹聚合物（MIP）修饰闭双极电极的电致化学发光（ECL）双模传感器平台，用于高灵敏度检测 L-Phe 和 D-苯丙氨酸（D-Phe）。在双极电极的阳极室中，经苯丙氨酸印迹修饰后，Ru（bpy）在驱动电压的刺激下发生氧化还原反应，产生化学发光响应。同时，双极电极阴极膜的还原得到促进，颜色从深蓝色变为近白色。因此，实现了目标分子的双模检测。该传感器对苯丙氨酸的检测范围达到 0.01-10000 nM，L-Phe 和 D-Phe 的检测限分别为 3.9 pM 和 4.6 pM（S/N = 3）。该双模系统实现了高稳定性和高特异性，还成功实现了实际样品的检测，有望实现未来的临床应用。
