Hajós F
Neurosci Lett. 1986 Nov 11;71(2):153-6. doi: 10.1016/0304-3940(86)90550-1.
Slices of 8-day-old rat cerebella were prepared with a tissue chopper, incubated in an oxygenated Krebs-Henseleit solution followed by processing for histology. One micrometer thick sections of resin-embedded slices were stained with Toluidine blue. The external granular layer was shown to maintain its normal proliferative activity up to 3 h of incubation as suggested by the presence of all mitotic phases within this period and by a roughly 50% increase in mitotic index upon a 90-min exposure of incubated slices to 20 microM vinblastine. On this basis the incubated slice of the immature rat cerebellum may serve as a simple tool for the rapid histological testing of short-term mitogenic or cytostatic effects and the vulnerability of a brain germinal layer.
用组织切片机切取8日龄大鼠小脑切片，置于充氧的克雷布斯 - 亨斯莱特溶液中孵育，随后进行组织学处理。将树脂包埋切片制成1微米厚的切片，用甲苯胺蓝染色。外部颗粒层在孵育3小时内保持其正常增殖活性，这可由该时期内所有有丝分裂阶段的存在以及孵育切片在暴露于20微摩尔长春花碱90分钟后有丝分裂指数大约增加50%表明。在此基础上，未成熟大鼠小脑的孵育切片可作为一种简单工具，用于快速组织学检测短期促有丝分裂或细胞抑制作用以及脑生发层的易损性。
