Garthwaite J, Woodhams P L, Collins M J, Balázs R
Dev Neurosci. 1980;3(2):90-9. doi: 10.1159/000112381.
The morphology of incubated slices of cerebellum from rats of various ages between birth and 70-90 days was studied using light and electron microscopy. The slices were prepared using a mechanical chopper and were incubated in Krebs solution for 1 1/2-2 h. Up to 8 days of age, cellular and subcellular damage in the slices was only rarely encountered. At 14 days, the number of pyknotic cells had increased, particularly at the cut edges of the slices, but otherwise the morphology resembled that of the cerebellum fixed in situ. By 21 days, pyknosis and swelling was more extensive, the most vulnerable neurones appearing to be the Purkinje cells and the inhibitory interneurones in the molecular layer, the internal granule cell layer being relatively spared. In slices from the adult, damage had further increased to include more than 90% of the granule cells. This non-uniform preservation of the cells in the slices with respect to age is likely to have a considerable influence on results from developmental studies carried out using mechanically-chopped brain slices. When prepared by hand, using a thin blade and glass guide, slices from the adult cerebellum showed good morphological preservation.
利用光学显微镜和电子显微镜研究了出生至70 - 90天不同年龄大鼠小脑孵育切片的形态学。切片用机械切片机制备，并在 Krebs 溶液中孵育1.5 - 2小时。在8日龄之前，切片中细胞和亚细胞损伤很少见。14日龄时，固缩细胞数量增加，特别是在切片的切割边缘，但在其他方面，其形态与原位固定的小脑相似。到21日龄时，固缩和肿胀更为广泛，最易受损的神经元似乎是浦肯野细胞和分子层中的抑制性中间神经元，内颗粒细胞层相对未受影响。在成年大鼠的切片中，损伤进一步增加，包括超过90%的颗粒细胞。切片中细胞相对于年龄的这种不均匀保存可能对使用机械切碎的脑切片进行的发育研究结果产生相当大的影响。当用薄刀片和玻璃导向器手工制备时，成年小脑的切片显示出良好的形态保存。
