Zhu Tianbo, Loyez Médéric, Chah Karima, Caucheteur Christophe
Opt Express. 2023 Sep 25;31(20):32478-32487. doi: 10.1364/OE.498571.
Surface plasmon resonance sensor based on gold-coated tilted fiber Bragg gratings (SPR-TFBGs) are perfectly suited for fine refractometry. Thanks to the functionalization of the gold layer, they can be used for label-free biosensing. They have been largely used for the specific detection of proteins and cells. In this work, we experimentally demonstrate that they are enough sensitive to detect a very small entity like an environmental pollutant. In this context, we report here a bio-functionalization of the SPR-TFBG with thrombin aptamers for lead ion detection. We used aqueous solutions of lead ions with increasing concentrations from 0.001 ppb to 10 ppb. Based on the affinity bending of Pb ions to the thrombin aptamer, we experimentally demonstrated low detection level of lead ion concentration (0.001 ppb) while the saturation limit is meanly fixed by the physical dimension of the sensor and the binding efficiency.
基于镀金黄倾斜光纤布拉格光栅（SPR-TFBG）的表面等离子体共振传感器非常适合精细折射测量。由于金层的功能化，它们可用于无标记生物传感。它们已被广泛用于蛋白质和细胞的特异性检测。在这项工作中，我们通过实验证明它们足够灵敏，能够检测像环境污染物这样非常小的实体。在此背景下，我们在此报告用凝血酶适体对SPR-TFBG进行生物功能化以检测铅离子。我们使用了浓度从0.001 ppb增加到10 ppb的铅离子水溶液。基于铅离子与凝血酶适体的亲和弯曲，我们通过实验证明了铅离子浓度的低检测水平（0.001 ppb），而饱和极限主要由传感器的物理尺寸和结合效率确定。
