School of Physics and Optoelectronic Technology, Dalian University of Technology, 2 Linggong Road, Ganjingzi District, Dalian 116024, China.
Sensors (Basel). 2017 Oct 1;17(10):2259. doi: 10.3390/s17102259.
We proposed and demonstrated a novel tilted fiber Bragg grating (TFBG)-based surface plasmon resonance (SPR) label-free biosensor via a special boronic acid derivative to detect glycoprotein with high sensitivity and selectivity. TFBG, as an effective sensing element for optical sensing in near-infrared wavelengths, possess the unique capability of easily exciting the SPR effect on fiber surface which coated with a nano-scale metal layer. SPR properties can be accurately detected by measuring the variation of transmitted spectra at optical communication wavelengths. In our experiment, a 10° TFBG coated with a 50 nm gold film was manufactured to stimulate SPR on a sensor surface. To detect glycoprotein selectively, the sensor was immobilized using designed phenylboronic acid as the recognition molecule, which can covalently bond with 1,2- or 1,3-diols to form five- or six-membered cyclic complexes for attaching diol-containing biomolecules and proteins. The phenylboronic acid was synthetized with long alkyl groups offering more flexible space, which was able to improve the capability of binding glycoprotein. The proposed TFBG-SPR sensors exhibit good selectivity and repeatability with a protein concentration sensitivity up to 2.867 dB/ (mg/mL) and a limit of detection (LOD) of 15.56 nM.
我们提出并展示了一种新颖的基于倾斜光纤布拉格光栅（TFBG）的表面等离子体共振（SPR）无标记生物传感器，通过特殊的硼酸衍生物来检测糖蛋白，具有高灵敏度和选择性。TFBG 作为近红外波长光学传感的有效传感元件，具有在涂覆纳米级金属层的光纤表面轻松激发 SPR 效应的独特能力。SPR 特性可以通过测量光通信波长下传输光谱的变化来准确检测。在我们的实验中，制造了一个涂有 50nm 金膜的 10° TFBG 来刺激传感器表面的 SPR。为了选择性地检测糖蛋白，传感器使用设计的苯硼酸作为识别分子进行固定，苯硼酸可以与 1,2-或 1,3-二醇共价键合形成五或六元环配合物，用于连接含二醇的生物分子和蛋白质。苯硼酸合成了带有长烷基基团的长烷基基团，提供了更多灵活的空间，从而能够提高与糖蛋白结合的能力。所提出的 TFBG-SPR 传感器具有良好的选择性和重复性，蛋白质浓度灵敏度高达 2.867dB/（mg/mL），检测限（LOD）为 15.56nM。
