Opt Express. 2023 Sep 25;31(20):33175-33190. doi: 10.1364/OE.498022.
Intracellular lasers are emerging as powerful biosensors for multiplexed tracking and precision sensing of cells and their microenvironment. This sensing capacity is enabled by quantifying their narrow-linewidth emission spectra, which is presently challenging to do at high speeds. In this work, we demonstrate rapid snapshot hyperspectral imaging of intracellular lasers. Using integral field mapping with a microlens array and a diffraction grating, we obtain images of the spatial and spectral intensity distribution from a single camera acquisition. We demonstrate widefield hyperspectral imaging over a 3 × 3 mm field of view and volumetric imaging over 250 × 250 × 800 µm (XYZ) volumes with a lateral (XY) resolution of 5 µm, axial (Z) resolution of 10 µm, and a spectral resolution of less than 0.8 nm. We evaluate the performance and outline the challenges and strengths of snapshot methods in the context of characterizing the emission from intracellular lasers. This method offers new opportunities for a diverse range of applications, including high-throughput and long-term biosensing with intracellular lasers.
细胞内激光器正成为用于对细胞及其微环境进行多路跟踪和精密感应的强大生物传感器。这种感应能力是通过量化其窄线宽发射光谱来实现的，而目前这在高速下具有挑战性。在这项工作中，我们展示了细胞内激光器的快速快照高光谱成像。我们使用微透镜阵列和衍射光栅进行积分场映射，从单个相机获取中获得空间和光谱强度分布的图像。我们展示了在 3×3 毫米视场的宽场高光谱成像和在 250×250×800 微米（XYZ）体积的体积成像，具有 5 微米的横向（XY）分辨率、10 微米的轴向（Z）分辨率和小于 0.8nm 的光谱分辨率。我们评估了该方法的性能，并概述了快照方法在表征细胞内激光器发射方面的挑战和优势。这种方法为包括使用细胞内激光器进行高通量和长期生物感应在内的各种应用提供了新的机会。
