Mongolian Medicine School, Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010110, P.R. China.
Inner Mongolia Key Laboratory of Disease‑Related Biomarkers, The Second Affiliated Hospital, Baotou Medical College, Baotou, Inner Mongolia Autonomous Region 014030, P.R. China.
Mol Med Rep. 2023 Dec;28(6). doi: 10.3892/mmr.2023.13115. Epub 2023 Oct 20.
Several studies have revealed that an imbalance of the intestinal microbiota is involved in intestinal inflammation associated with ulcerative colitis (UC). Therefore, regulating the homeostasis of gut microbiota is critical for treating UC. (DML) extract, a common traditional Chinese medicine, has been demonstrated to possess numerous pharmacological effects, such as antioxidative, anti‑inflammatory, and antibacterial properties. The aim of the present study was to evaluate the beneficial effects of DML extract and the probable mechanism of action in a dextran sulfate sodium‑induced chronic colitis model. It was found that DML extract ameliorated UC by improving disease activity index, weight loss, colon length, and histological scoring. DML extract administration also enhanced the count of and reduced the count of . Furthermore, the results of network pharmacology analysis revealed that the active ingredients (including luteolin, rosmarinic acid, oleanolic acid, ursolic acid, apigenin, acacetin, kaempferol, and isorhamnetin) in the DML extract were closely associated with anti‑inflammatory activity via various signaling pathways, including the NF‑κB, IL‑17, TNF, and Toll‑like receptor (TLR) signaling pathways. Western blot analysis further indicated that DML extract downregulated the expression of members of the TLR4/NF‑κB signaling pathway, which was associated with colitis. Thus, it was hypothesized that DML extract exerted its anti‑colitis effects by modulating the gut microbiota and inflammatory pathways.
几项研究表明，肠道微生物群的失衡与溃疡性结肠炎（UC）相关的肠道炎症有关。因此，调节肠道微生物群的平衡对于治疗 UC 至关重要。（DML）提取物是一种常见的中药，已被证明具有多种药理作用，如抗氧化、抗炎和抗菌特性。本研究旨在评估 DML 提取物在葡聚糖硫酸钠诱导的慢性结肠炎模型中的有益作用及其可能的作用机制。结果发现，DML 提取物通过改善疾病活动指数、体重减轻、结肠长度和组织学评分来改善 UC。DML 提取物给药还增加了 的数量，减少了 的数量。此外，网络药理学分析的结果表明，DML 提取物中的活性成分（包括木犀草素、迷迭香酸、齐墩果酸、熊果酸、芹菜素、木犀草素、山奈酚和异鼠李素）通过各种信号通路（包括 NF-κB、IL-17、TNF 和 Toll 样受体（TLR）信号通路）与抗炎活性密切相关。Western blot 分析进一步表明，DML 提取物下调了与结肠炎相关的 TLR4/NF-κB 信号通路成员的表达。因此，研究人员假设 DML 提取物通过调节肠道微生物群和炎症途径发挥其抗结肠炎作用。
