Quinn M A, Clyne J H, Wolf M M, Cruickshank D, Cooper I A, McGrath K M, Morris J
Pathology. 1986 Jul;18(3):331-5. doi: 10.3109/00313028609059486.
Four types of plastic blood collection packs were studied for their ability to preserve platelet function during a 5 d storage period. The platelet concentrates were stored in polyvinyl chloride (Tuta Laboratories), PL 1240 and PL 732 (Fenwal Laboratories) and CLX (Cutter Laboratories) packs, on a Fenwal elliptical rotator at 20 degrees-24 degrees C. Plasma pH, lactate concentration, hypotonic shock response (HSR), platelet aggregation in response to ADP, collagen and ristocetin and levels of the plasticisers, di-2-ethylhexyl phthalate (DEHP) and tri-ethylhexyl trimellitate (TEHTM), were measured. Morphological changes were assessed by electron microscopy. No significant fall in pH occurred in any type of pack but in vitro function and platelet morphology was generally better preserved in Tuta and CLX packs than in PL 732 and PL 1240. Very little TEHTM leached out of the PL 1240 and CLX packs whereas the mean concentration of DEHP in the platelet concentrates stored in Tuta packs was 27.4 mg/100 ml plasma after 5 d of storage. The results indicate that it is possible to prepare and store platelet concentrates in polyvinyl chloride plastic packs for a period of 5 d and maintain their function and viability.
研究了四种塑料采血袋在5天储存期内保存血小板功能的能力。血小板浓缩物分别储存在聚氯乙烯袋（图塔实验室）、PL 1240袋和PL 732袋（芬瓦尔实验室）以及CLX袋（卡特实验室）中，置于芬瓦尔椭圆旋转器上，温度为20摄氏度至24摄氏度。测量了血浆pH值、乳酸浓度、低渗休克反应（HSR）、对ADP、胶原和瑞斯托菌素的血小板聚集以及增塑剂邻苯二甲酸二（2-乙基己基）酯（DEHP）和偏苯三酸三（乙基己基）酯（TEHTM）的水平。通过电子显微镜评估形态学变化。任何类型的袋子中pH值均未出现显著下降，但图塔袋和CLX袋中的体外功能和血小板形态总体上比PL 732袋和PL 1240袋保存得更好。PL 1240袋和CLX袋中渗出的TEHTM极少，而储存5天后，储存在图塔袋中的血小板浓缩物中DEHP的平均浓度为27.4毫克/100毫升血浆。结果表明，有可能在聚氯乙烯塑料袋中制备和储存血小板浓缩物5天，并保持其功能和活力。
