Chokeshaiusaha Kaj, Sananmuang Thanida, Puthier Denis, Nguyen Catherine
Department of Veterinary Science, Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-OK, Chonburi, Thailand.
Aix-Marseille Université, INSERM, UMR 1090, TAGC, Marseille, France.
Vet World. 2023 Sep;16(9):1964-1973. doi: 10.14202/vetworld.2023.1964-1973. Epub 2023 Sep 23.
Fatty liver disease is a common condition, characterized by excess fat accumulation in the liver. It can contribute to more severe liver-related health issues, making it a critical concern in avian and human medicine. Apart from modifying the gene expression of liver cells, the disease also alters the expression of specific transcript isoforms, which might serve as new biological markers for both species. This study aimed to identify cross-species genes displaying differential expressions in their transcript isoforms in humans and chickens with fatty liver disease.
We performed differential gene expression and differential transcript usage (DTU) analyses on messenger RNA datasets from the livers of both chickens and humans with fatty liver disease. Using appropriate cross-species gene identification methods, we reviewed the acquired candidate genes and their transcript isoforms to determine their potential role in fatty liver disease's pathogenesis.
We identified seven genes - , , , , , , , and - and their corresponding transcript isoforms as potential candidates (false discovery rate ≤0.05). Our findings showed that these genes most likely contribute to fatty disease development and progression.
This study successfully identified novel human-chicken DTU genes in fatty liver disease. Further research is encouraged to verify the functions and regulations of these transcript isoforms as potential diagnostic markers for fatty liver disease in humans and chickens.
脂肪肝疾病是一种常见病症，其特征为肝脏中脂肪过度蓄积。它可能导致更严重的肝脏相关健康问题，这使其成为禽类和人类医学中的一个关键关注点。除了改变肝细胞的基因表达外，该疾病还会改变特定转录本异构体的表达，这可能成为这两个物种的新生物标志物。本研究旨在鉴定在患有脂肪肝疾病的人类和鸡中，其转录本异构体表现出差异表达的跨物种基因。
我们对患有脂肪肝疾病的鸡和人类肝脏的信使核糖核酸数据集进行了差异基因表达和差异转录本使用（DTU）分析。使用适当的跨物种基因鉴定方法，我们审查了获得的候选基因及其转录本异构体，以确定它们在脂肪肝疾病发病机制中的潜在作用。
我们鉴定出七个基因—— 、 、 、 、 、 、 以及 ，及其相应的转录本异构体为潜在候选基因（错误发现率≤0.05）。我们的研究结果表明，这些基因很可能促成了脂肪疾病的发展和进展。
本研究成功鉴定出了脂肪肝疾病中新型的人鸡DTU基因。鼓励进一步研究以验证这些转录本异构体作为人类和鸡脂肪肝疾病潜在诊断标志物的功能和调控机制。
