Alseady Haider H, Al-Dabbagh Sahad M K, Marhash Ali D
Technical Institute of Babylon, Al-Furat Al-Awsat Technical University, 51015, Babylon, Iraq.
Institute of Medical Technology Al-Mansour, Middle Technical University, 10001, Baghdad, Iraq.
Vet World. 2023 Sep;16(9):1781-1789. doi: 10.14202/vetworld.2023.1781-1789. Epub 2023 Sep 13.
is one of the most prevalent intestinal parasites in humans and animals, and children in close contact with livestock are particularly at risk of infection. This study aimed to detect assemblages of and determine the origin of zoonotic transmission of in children and calves in different parts of Babylon province, Iraq.
One hundred stool samples from children (68 boys and 32 girls) and 100 fecal samples from calves (46 males and 54 females) of different ages were randomly collected. Molecular techniques were used to estimate the prevalence of in children and calves. A nested polymerase chain reaction (PCR) was performed by targeting the triose phosphate isomerase gene in the samples to detect assemblages.
The overall rates of infection with in children and calves were 21% and 34%, respectively, using the conventional microscopic method. The results illustrated that 61.90% (13/21) and 38.09% (8/21) of positive samples from children were allocated to assemblages A and B, respectively (p > 0.05). In calves, assemblages A and B were detecte in 82.35% (28/34) and 17.64% (6/34) of positive samples from calves, respectively (p ≤ 0.001). Ten PCR products were sequenced and submitted to the GenBank database. Phylogenetic analysis detected five human sequences each belonging to assemblages A (OM850335-OM850339) and B (OM850340-OM850344). Similarly, five calf sequences each belonged to assemblages A (ON75756-ON757660) and B (ON757661-ON757665).
The detection of large numbers of assemblage A in both humans and cattle indicated that cattle could be a main source of zoonotic infection in children in Babylon province, Iraq.
[寄生虫名称]是人和动物中最常见的肠道寄生虫之一，与家畜密切接触的儿童感染风险尤其高。本研究旨在检测伊拉克巴比伦省不同地区儿童和小牛体内[寄生虫名称]的组合，并确定其人畜共患传播的来源。
随机收集100份不同年龄段儿童（68名男孩和32名女孩）的粪便样本和100份不同年龄段小牛（46头雄性和54头雌性）的粪便样本。采用分子技术估计儿童和小牛体内[寄生虫名称]的感染率。通过针对样本中的磷酸丙糖异构酶基因进行巢式聚合酶链反应（PCR）来检测[寄生虫名称]的组合。
使用传统显微镜方法，儿童和小牛体内[寄生虫名称]的总体感染率分别为21%和34%。结果表明，儿童阳性样本中分别有61.90%（13/21）和38.09%（8/21）被分配到组合A和组合B（p>0.05）。在小牛中，组合A和组合B分别在82.35%（28/34）和17.64%（6/34）的小牛阳性样本中被检测到（p≤0.001）。对10个PCR产物进行测序并提交到GenBank数据库。系统发育分析检测到5个人类序列，每个序列分别属于[寄生虫名称]组合A（OM850335 - OM850339）和组合B（OM850340 - OM850344）。同样，5个小牛序列分别属于[寄生虫名称]组合A（ON75756 - ON757660）和组合B（ON757661 - ON757665）。
在人和牛中均检测到大量的[寄生虫名称]组合A，这表明牛可能是伊拉克巴比伦省儿童人畜共患[寄生虫名称]感染的主要来源。
