Kashinahanji Manizheh, Haghighi Ali, Bahrami Fares, Fallah Mohammad, Saidijam Masoud, Matini Mohammad, Maghsood Amir Hossein
1Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
2Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Parasit Dis. 2019 Dec;43(4):616-623. doi: 10.1007/s12639-019-01139-x. Epub 2019 Jul 1.
is a very abundant organism bringing about diarrhoea in human beings. The focus of this analysis was the detection of assemblages in human stool specimens in Hamadan, west of Iran, as well as the association between obtained assemblages and clinical symptoms. Faecal samples of 4066 individuals admitted to the medical and health care facilities in Hamadan were inspected microscopically for the existence of cysts/trophozoites, and the clinical symptoms of the patients were recorded. The DNA of positive samples was isolated from and the nucleotide sequences of both glutamate dehydrogenase () (n = 15) and triose phosphate isomerase () (n = 8) genes were analyzed. In direct microscopy, a total of sixty-four samples (1.6%), were considered as positive for cysts or trophozoites. The sequence analysis showed that 18 out of 23 sequenced isolates (78.2%) were assemblage A and 5 (21.7%) were assemblage B. Clinical symptoms were observed in 44.4% and 40% of patients with assemblages A and B, respectively. Overall, the predominant assemblage A detected in the tested samples along with bioinformatics analysis suggest a potential zoonotic transmission in the region of the study. Although advanced analyses are necessary to understand the foundation and path of the infection, it seems that more sanitary regulations regarding contact with livestock and pet animals are essential.
是一种在人类中引发腹泻的极为常见的生物体。本分析的重点是检测伊朗西部哈马丹人类粪便样本中的菌群组合，以及所获得的菌群组合与临床症状之间的关联。对哈马丹医疗保健机构收治的4066名个体的粪便样本进行显微镜检查，以确定是否存在囊肿/滋养体，并记录患者的临床症状。从阳性样本中分离出DNA，并分析谷氨酸脱氢酶（n = 15）和磷酸丙糖异构酶（n = 8）基因的核苷酸序列。在直接显微镜检查中，共有64个样本（1.6%）被认为囊肿或滋养体呈阳性。序列分析表明，23个测序分离株中有18个（78.2%）为A群，5个（21.7%）为B群。A群和B群患者的临床症状发生率分别为44.4%和40%。总体而言，在所检测样本中检测到的主要A群以及生物信息学分析表明，该研究区域存在潜在的人畜共患病传播。尽管需要进行进一步分析以了解感染的基础和途径，但似乎有必要制定更多关于接触家畜和宠物的卫生规定。
