Rushidi Mohd Nur Azad, Azhari Muhammad Luqman Hakim, Yaakop Salmah, Hazmi Izfa Riza
Centre for Insect Systematics, Department of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Trop Life Sci Res. 2023 Sep;34(3):95-111. doi: 10.21315/tlsr2023.34.3.5. Epub 2023 Sep 30.
is the most efficient pollinator of oil palm. is an endosymbiotic bacteria associated with that has a potential to affect the fecundity and fitness of the . Despite their importance, no studies have been conducted to investigate its prevalence in . The objectives of this study were to detect and characterise in and determine the phylogenetic relationship of strains that infect by using three genetic markers namely Filamenting temperature-sensitive mutant Z (), Chaperonin folding protein (), and Citrate Synthase Coding Gene (). DNA was extracted from 210 individuals of and the infections were detected using the marker. The infected samples ( = 25, 11.9%) were then sequenced using and markers for strain characterization. In this study, a combination of four markers was used to construct the phylogeny of . Similar topologies were shown in all trees; Neighbour-Joining (NJ), Maximum Parsimony (MP), and Bayesian Inference (BI), which showed the mixing of individuals that harbor between populations. Interestingly, on was claded together with the species under supergroup B. This is the first report of infecting which is very valuable and significant as one of the parameters to evaluate the quality of the population for sustaining its function as a great pollinator for oil palm.
是油棕最有效的传粉者。是一种与相关的内共生细菌，它有可能影响的繁殖力和适合度。尽管它们很重要，但尚未开展研究来调查其在中的流行情况。本研究的目的是检测和鉴定中的，并通过使用丝状温度敏感突变体Z（）、伴侣蛋白折叠蛋白（）和柠檬酸合酶编码基因（）这三个遗传标记来确定感染的菌株的系统发育关系。从210个个体中提取DNA，并使用标记检测感染情况。然后使用和标记对感染样本（=25，11.9%）进行测序以进行菌株鉴定。在本研究中，使用四个标记的组合来构建的系统发育树。在所有树中都显示出相似的拓扑结构；邻接法（NJ）、最大简约法（MP）和贝叶斯推断法（BI），这些方法显示了不同种群中携带的个体的混合情况。有趣的是，上的与超群B下的物种聚集在一起。这是关于感染的首次报道，作为评估种群质量以维持其作为油棕重要传粉者功能的参数之一，这非常有价值且意义重大。
