Shangguan Yuchen, Ni Jing, Jiang Lili, Hu Yang, He Chuanbo, Ma Ying, Wu Guohong, Xiong Hejian
College of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, 361021, China.
Jiangle County Agricultural Products Quality and Safety Inspection Station, Sanming, 353300, China.
Curr Res Food Sci. 2023 Oct 5;7:100610. doi: 10.1016/j.crfs.2023.100610. eCollection 2023.
In this study, flavonoids were extracted from pomelo peels and naringin was isolated from the flavonoid extract. The effects of extraction parameters, namely, ethanol concentration, solid-to-liquid ratio, and extraction time, on the yield of flavonoids extracted from pomelo peels were analyzed according to the Box-Behnken design of response surface methodology. The experimental conditions for flavonoid extraction were optimized, and naringin was separated from the extracted flavonoids using Sephadex LH-20 column chromatography. Experimental results showed that the influence of factors on the extraction rate of flavonoids from pomelo peels was in the order of ethanol concentration > solid-to-liquid ratio > extraction time, and the optimal extraction parameters were 85% ethanol concentration, 1:20 solid-to-liquid ratio, and 4-h extraction time for extracting flavonoids from pomelo peels. Under these conditions, the yield of flavonoids was 6.07 ± 0.06 mg/g. After three times of extraction, the flavonoid extraction rate reached 96.55%, and the residual naringin in the pomelo peels was 0.017 mg/g, at which point the bitterness in the pomelo peels disappeared. Two components, namely, PF1 and PF2, were separated from the crude flavonoid of pomelo peels through Sephadex LH20 column chromatography. PF2 was identified as naringin by high-performance liquid chromatography tandem mass spectrometry, with a purity of 95.7 ± 0.23%. Both flavonoids and PF2 exhibited good in vitro radicals scavenging activities on DPPH, ABTS, superoxide anion and hydroxyl.
在本研究中，从柚子皮中提取黄酮类化合物，并从黄酮类提取物中分离出柚皮苷。根据响应面法的Box-Behnken设计，分析了提取参数（即乙醇浓度、固液比和提取时间）对从柚子皮中提取黄酮类化合物产量的影响。优化了黄酮类化合物的提取条件，并使用Sephadex LH-20柱色谱从提取的黄酮类化合物中分离出柚皮苷。实验结果表明，各因素对柚子皮黄酮类化合物提取率的影响顺序为乙醇浓度＞固液比＞提取时间，从柚子皮中提取黄酮类化合物的最佳提取参数为乙醇浓度85%、固液比1:20、提取时间4 h。在此条件下，黄酮类化合物的产量为6.07±0.06 mg/g。经过三次提取后，黄酮类化合物的提取率达到96.55%，柚子皮中残留的柚皮苷为0.017 mg/g，此时柚子皮中的苦味消失。通过Sephadex LH20柱色谱从柚子皮粗黄酮中分离出PF1和PF2两个组分。通过高效液相色谱串联质谱法鉴定PF2为柚皮苷，纯度为95.7±0.23%。黄酮类化合物和PF2在体外对DPPH、ABTS、超氧阴离子和羟基均表现出良好的自由基清除活性。
Zotero 插件
