Chandra Phool, Kaleem Mohammad, Sachan Neetu, Pathak Rashmi, Alanazi Ashwag S, Alsaif Nawaf A, Alsanea Sary, Alsuwayt Bader, Alanazi Mohammed M, Kabra Atul
Teerthanker Mahaveer College of Pharmacy, Teerthanker Mahaveer University, Moradabad, U.P. 244001, India.
School of Pharmaceutical Sciences, IFTM University, Lodhipur Rajput, Delhi Road (NH-24), Moradabad 244 102, U.P., India.
Saudi Pharm J. 2023 Nov;31(11):101815. doi: 10.1016/j.jsps.2023.101815. Epub 2023 Oct 5.
Traditional uses for the plant () (Alfalfa) (Family: Fabaceae) include liver protection, antioxidant activity, and the treatment of bleeding and digestive issues. This study aims to assess the effect of ethanol extract of (EEMS) on experimental-induced ulcers in diabetic rats. By pylorus ligation and ethanol administration, gastric ulcers were induced in diabetic rats. Five groups each consisting of six rats in each model were used. All other groups except Group I were made diabetic by giving rats alloxan (140 mg/kg i.p.). Vehicles were given to Group I (normal control) and Group II (diabetes control) rats. Group III (positive control) received ranitidine 50 mg/kg, and Group IV and V received EEMS at doses of 100 and 400 mg/kg, respectively. In the pylorus ligation and ethanol-induced stomach ulcer model of rats, the findings demonstrated that EEMS (100 mg/kg) showed a decreased ulcer index of 2.01 ± 0.41 and was found statistically significant against the diabetes control group (p < 0.001) as well as, an ulcer index of 0.68 ± 0.22 by EEMS (400 mg/kg) with a significant reduction in the ulcer index (p < 0.001). EEMS (100 and 400 mg/kg) reduce free acidity by 13.16 ± 0.65 mEq/L and 9.83 ± 0.30 mEq/L, respectively. EEMS also showed a protective impact on the liver and kidneys of diabetic rats. Antihyperglycemic action was also discovered in diabetic animals. The findings of the current investigation demonstrated that ethanolic extract of possesses anti-ulcer activity in diabetic rats. Ethanolic extract of may be a treatment option for stomach ulcers that also have diabetes.
植物紫花苜蓿（苜蓿属，豆科）的传统用途包括保护肝脏、抗氧化活性以及治疗出血和消化问题。本研究旨在评估紫花苜蓿乙醇提取物（EEMS）对糖尿病大鼠实验性溃疡的影响。通过幽门结扎和给予乙醇，在糖尿病大鼠中诱导胃溃疡。每个模型使用五组，每组六只大鼠。除第一组外，所有其他组大鼠通过腹腔注射四氧嘧啶（140 mg/kg）制成糖尿病模型。第一组（正常对照组）和第二组（糖尿病对照组）大鼠给予赋形剂。第三组（阳性对照组）接受50 mg/kg雷尼替丁，第四组和第五组分别接受100 mg/kg和400 mg/kg剂量的EEMS。在大鼠幽门结扎和乙醇诱导的胃溃疡模型中，研究结果表明，EEMS（100 mg/kg）的溃疡指数降低至2.01±0.41，与糖尿病对照组相比具有统计学意义（p<0.001），EEMS（400 mg/kg）的溃疡指数为0.68±0.22，溃疡指数显著降低（p<0.001）。EEMS（100和400 mg/kg）分别使游离酸度降低13.16±0.65 mEq/L和9.83±0.30 mEq/L。EEMS对糖尿病大鼠的肝脏和肾脏也显示出保护作用。在糖尿病动物中还发现了降血糖作用。本研究结果表明，紫花苜蓿乙醇提取物对糖尿病大鼠具有抗溃疡活性。紫花苜蓿乙醇提取物可能是患有糖尿病的胃溃疡患者的一种治疗选择。
