Shin Daeun, Ha Eunsu, Kong Minsuk, Ryu Sangryeol
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826 Korea.
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, 01811 Korea.
Food Sci Biotechnol. 2023 May 24;32(14):2069-2077. doi: 10.1007/s10068-023-01314-0. eCollection 2023 Dec.
is one of the major foodborne pathogens in humans and animals. With the prevalence of antibiotic-resistant strains, bacteriophages and their endolysins have received considerable attention as promising alternatives to antibiotics. In this study, phage CPD2 was isolated from retail chicken samples. CPD2 belongs to the family and exhibits remarkable thermostability. While CPD2 has narrow host specificity, its endolysin LysCPD2 showed a broader lytic range, killing not only strains but other Gram-positive bacteria, such as and . In addition, due to its exceptional thermal stability, LysCPD2 showed significant antibacterial ability against germinating spores during the heat activation process (75 °C for 20 min). Taken together, these results indicate that both thermostable phage CPD2 and its endolysin LysCPD2 can be used as efficient antimicrobial agents to control during thermal processing of foods.
是人和动物中主要的食源性病原体之一。随着抗生素耐药菌株的流行，噬菌体及其内溶素作为抗生素的有前景的替代品受到了相当大的关注。在本研究中，噬菌体CPD2是从零售鸡肉样本中分离出来的。CPD2属于 科，具有显著的热稳定性。虽然CPD2宿主特异性狭窄，但其内溶素LysCPD2显示出更广泛的裂解范围，不仅能杀死 菌株，还能杀死其他革兰氏阳性细菌，如 和 。此外，由于其卓越的热稳定性，LysCPD2在热激活过程（75°C 20分钟）中对萌发的 孢子显示出显著的抗菌能力。综上所述，这些结果表明，热稳定噬菌体CPD2及其内溶素LysCPD2均可作为有效的抗菌剂，用于食品热加工过程中控制 。
