Souid Moufida, Bday Jaweher, Souissi Sameh, Ghedira Randa, Gabbouj Sallouha, Shini-Hadhri Salwa, Toumi Dhekra, Bergaoui Haifa, Zouari Ines, Faleh Raja, Zakhama Abdelfatteh, Hassen Elham
Molecular Immuno-Oncology Laboratory, Monastir University, Monastir, Tunisia.
Higher Institute of Biotechnology of Monastir, Monastir University, Monastir, Tunisia.
Biomarkers. 2023 Dec;28(7):628-636. doi: 10.1080/1354750X.2023.2273226. Epub 2023 Dec 11.
The implication of arginase enzyme in Human Papillomavirus (HPV) infections has not been clearly elucidated. The present study investigates whether HPV infection is correlated with changes in plasmatic arginase activity and cervical ARG1 and ARG2 mRNA expression among infected women negative for intraepithelial lesions (NIL).
The present study included 300 women. The plasmatic arginase activity was evaluated by a colorimetric assay. Cervical HPV was detected by real-time PCR. The circulating viral load and ARG1 and ARG2 mRNA expression quantification were performed by quantitative real-time PCR.
A significant increase in plasma arginase activity and ARG1 and ARG2 mRNA expression levels in cervical cells was observed among HPV-positive women compared to the HPV-negative group. The highest levels were significantly associated with oncogenic HPV, and increased arginase activity was associated with a high HPV circulating viral load. Moreover, the highest levels of arginase activity were observed in oncogenic HPV-positive inflammatory smears.
These data suggest that HPV could modulate arginase activity and expression, which may restrict arginine bioavailability and inhibit this amino acid's antiviral properties.
Our findings revealed that arginase activity and isoform gene expression were upregulated in women with HPV infection, particularly the oncogenic HPV types.
精氨酸酶在人乳头瘤病毒（HPV）感染中的作用尚未完全阐明。本研究调查HPV感染是否与上皮内病变阴性（NIL）感染女性的血浆精氨酸酶活性变化以及宫颈ARG1和ARG2 mRNA表达相关。
本研究纳入300名女性。采用比色法评估血浆精氨酸酶活性。通过实时PCR检测宫颈HPV。通过定量实时PCR进行循环病毒载量以及ARG1和ARG2 mRNA表达定量分析。
与HPV阴性组相比，HPV阳性女性的血浆精氨酸酶活性以及宫颈细胞中ARG1和ARG2 mRNA表达水平显著升高。最高水平与致癌性HPV显著相关，精氨酸酶活性增加与高HPV循环病毒载量相关。此外，在致癌性HPV阳性炎性涂片样本中观察到最高水平的精氨酸酶活性。
这些数据表明HPV可能调节精氨酸酶活性和表达，这可能会限制精氨酸的生物利用度并抑制该氨基酸的抗病毒特性。
我们的研究结果显示，HPV感染女性，尤其是致癌性HPV类型感染的女性，精氨酸酶活性和同工型基因表达上调。
