Manavi M, Hudelist G, Fink-Retter A, Gschwantler-Kaulich D, Pischinger K, Czerwenka K
Division of Special Gynecology, Department of Gynecology and Obstetrics, Medical University of Vienna, Vienna, Austria.
Int J Gynecol Cancer. 2008 Mar-Apr;18(2):285-94. doi: 10.1111/j.1525-1438.2007.01017.x. Epub 2007 Jun 22.
The human papillomavirus (HPV) plays an important role in the progression of cervical carcinoma. High-risk (HR) HPV types have been mainly identified in cytologic high-grade squamous intraepithelial lesions (HSILs) and histologic invasive carcinoma of the cervix. We examined cervical swabs of patients with abnormal Papanicolaou (Pap) smears, diagnosed as low-grade squamous intraepithelial lesions (LSILs) including atypical squamous cells of uncertain significance or HSILs. Low-risk (LR) HPV and HR-HPV types were identified by the Digene Hybrid Capture II test. Two-dimensional (2D) gel electrophoresis was used to specify the physical state of HPV DNA sequences. Expression of E6/E7 messenger RNA (mRNA) transcripts was analyzed by reverse transcriptase-polymerase chain reaction. Histopathologic results were correlated to the patients' physical status and HPV DNA mRNA transcripts. Pap smears with HPV infections of LR and HR types were correlated to the degree of squamous intraepithelial lesions (SILs). Comparing the physical states of HPV DNA sequences with the expression of HPV E6/E7 mRNA transcripts, all types were identified only as extrachromosomal in benign cervical smears, cervical intraepithelial neoplasia (CIN) I and II. HPV16 showed all physical states in CIN III/carcinoma in situ (CIS), whereas HPV18 only existed in mixed and integrated forms. HPV31/33/52b/58 appeared in all stages of lesions most commonly in extrachromosomal form; in integrated form, they were present only in CIN III/CIS. Although integration of some HR-HPV types is not always necessary for progression of SILs, the above-mentioned method is useful to analyze the physical state of HPV DNA sequences and predict the progression of SILs.
人乳头瘤病毒（HPV）在宫颈癌的发展过程中起着重要作用。高危（HR）HPV类型主要在细胞学高级别鳞状上皮内病变（HSIL）和宫颈组织学浸润癌中被发现。我们检查了巴氏涂片异常的患者的宫颈拭子，这些患者被诊断为低级别鳞状上皮内病变（LSIL），包括意义不明确的非典型鳞状细胞或HSIL。通过Digene杂交捕获II试验鉴定低危（LR）HPV和HR - HPV类型。二维（2D）凝胶电泳用于确定HPV DNA序列的物理状态。通过逆转录聚合酶链反应分析E6/E7信使核糖核酸（mRNA）转录物的表达。组织病理学结果与患者的身体状况以及HPV DNA mRNA转录物相关。LR和HR型HPV感染的巴氏涂片与鳞状上皮内病变（SIL）的程度相关。将HPV DNA序列的物理状态与HPV E6/E7 mRNA转录物的表达进行比较，所有类型在良性宫颈涂片、宫颈上皮内瘤变（CIN）I和II中仅被鉴定为染色体外形式。HPV16在CIN III/原位癌（CIS）中显示出所有物理状态，而HPV18仅以混合和整合形式存在。HPV31/33/52b/58在病变的所有阶段最常以染色体外形式出现；以整合形式存在时，它们仅出现在CIN III/CIS中。尽管某些HR - HPV类型的整合对于SIL的进展并非总是必要的，但上述方法有助于分析HPV DNA序列的物理状态并预测SIL的进展。
