Department of Spine Surgery and Musculoskeletal Tumor, Zhongnan Hospital of Wuhan University, Wuhan, China.
Clin Exp Med. 2023 Dec;23(8):5389-5398. doi: 10.1007/s10238-023-01216-9. Epub 2023 Oct 20.
Heat shock protein B6 (HSPB6) plays a certain role in the formation of several cancers, whereas its effect on osteosarcoma remains unclear. In this study, the effect of HSPB6 on osteosarcoma was validated through numerous experiments. HSPB6 was down-regulated in osteosarcoma. As indicated by the result of CCK-8 and colony formation assays, HSPB6 overexpression was likely to inhibit the osteosarcoma cells proliferation, whereas the flow cytometry analysis suggested that apoptosis of osteosarcoma cells was increased after HSPB6 overexpression. Furthermore, transwell and wound healing assays suggested that when HSPB6 was overexpressed, osteosarcoma cells migration and invasion were declined. Moreover, the western blotting assay suggested that the protein level of p-ERK1/2 was down-regulated in osteosarcoma when HSPB6 was overexpressed. Besides, the effect of HSPB6 on osteosarcoma in vivo was examined. As indicated by the result, HSPB6 overexpression was likely to prevent osteosarcoma growth and lung metastasis in vivo. As revealed by the findings of this study, HSPB6 overexpression exerted anticancer effects in osteosarcoma through the ERK signaling pathway and HSPB6 may be suitable target for osteosarcoma molecular therapies.
热休克蛋白 B6（HSPB6）在几种癌症的形成中发挥一定作用，但其对骨肉瘤的影响尚不清楚。在这项研究中，通过多项实验验证了 HSPB6 对骨肉瘤的影响。HSPB6 在骨肉瘤中下调。CCK-8 和集落形成实验的结果表明，HSPB6 过表达可能抑制骨肉瘤细胞的增殖，而流式细胞术分析表明 HSPB6 过表达后骨肉瘤细胞凋亡增加。此外，Transwell 和划痕愈合实验表明，当 HSPB6 过表达时，骨肉瘤细胞的迁移和侵袭能力下降。此外，Western blot 实验表明，HSPB6 过表达时骨肉瘤中 p-ERK1/2 的蛋白水平下调。此外，还检查了 HSPB6 对体内骨肉瘤的影响。结果表明，HSPB6 过表达可能预防体内骨肉瘤的生长和肺转移。本研究结果表明，HSPB6 过表达通过 ERK 信号通路对骨肉瘤发挥抗癌作用，HSPB6 可能是骨肉瘤分子治疗的合适靶点。
