Department of Biochemistry, Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, 04023-901, Brazil.
Laboratory of Herpetology, Instituto Butantan, São Paulo, Brazil.
Biochem Biophys Res Commun. 2023 Nov 26;683:149090. doi: 10.1016/j.bbrc.2023.10.022. Epub 2023 Oct 6.
Snake venoms are known to be major sources of peptides with different pharmacological properties. In this study, we comprehensively explored the venom peptidomes of three specimens of Lachesismuta, the largest venomous snake in South America, using mass spectrometry techniques. The analysis revealed 19 main chromatographic peaks common to all specimens. A total of 151 peptides were identified, including 69 from a metalloproteinase, 58 from the BPP-CNP precursor, and 24 from a l-amino acid oxidase. To our knowledge, 126 of these peptides were reported for the first time in this work, including a new SVMP-derived peptide fragment, Lm-10a. Our findings highlight the dynamic nature of toxin maturation in snake venoms, driven by proteolytic processing, post-translational modifications, and cryptide formation.
蛇毒是具有不同药理特性的肽类的主要来源。在这项研究中，我们使用质谱技术全面研究了三种南美的最大毒蛇——Lachesismuta 的毒液肽组。分析揭示了所有标本共有的 19 个主要色谱峰。共鉴定出 151 种肽，其中 69 种来自金属蛋白酶，58 种来自 BPP-CNP 前体，24 种来自 l-氨基酸氧化酶。据我们所知，其中 126 种肽在这项工作中是首次报道的，包括一个新的 SVMP 衍生肽片段 Lm-10a。我们的研究结果强调了毒素成熟在蛇毒液中的动态特性，这是由蛋白水解加工、翻译后修饰和隐蔽肽形成驱动的。
