Pang Wei Quan, Lai Chern Shun, Mad' Atari Mohamad Fadhli, Pandian Bothi Raja, Mohamad Ibrahim Mohamad Nasir, Tan Swee Tiam, Yoong Ivan Chew Ken, Subramaniam Sreeramanan
School of Biological Sciences, Universiti Sains Malaysia (USM), Georgetown, 11800, Penang, Malaysia.
School of Chemical Sciences, Universiti Sains Malaysia (USM), Georgetown, Penang, 11800, Malaysia.
Plant Physiol Biochem. 2023 Nov;204:108104. doi: 10.1016/j.plaphy.2023.108104. Epub 2023 Oct 16.
Graphene oxide (GO) is a novel nanomaterial with distinct physical properties and significant biological applications. The use of GO in plant tissue culture offers several new properties and potential applications. This research is vital due to the growing need for innovative techniques to promote plant growth, improve plant productivity and mitigate challenges posed by environmental stressors. This study focused on the rare Cameron Highlands white strawberry plants (Fragaria x ananassa) and addressed issues such as callus production during direct shoot induction and hyperhydricity. The research aimed to investigate the effects of GO on the regeneration process and genetic stability of white strawberry plants and to use molecular markers to ensure that plants propagated in vitro are true to type. For this purpose, shoot tip explants were used and different concentrations of GO (0, 2.5, 5.0, 7.5, 10 mg/L) were added to the Murashige and Skoog (MS) medium for six weeks. The results showed that the optimum concentration for promoting the development of white strawberry seedlings was 7.5 mg/L of GO. The study also revealed that the addition of 7.5 mg/L GO in combination with 8 μM TDZ to the MS medium facilitated the induction of multiple shoots. Moreover, the clonal fidelity of the in vitro plants treated with GO showed a genetic similarity of over 97%. These results confirm that lower GO concentrations improve plant development and stability. Consequently, this nanomaterial has a positive effect on the growth of strawberry plants and is therefore well suited for strawberry tissue culture.
氧化石墨烯（GO）是一种具有独特物理性质和重要生物学应用的新型纳米材料。GO在植物组织培养中的应用具有若干新特性和潜在应用。由于对促进植物生长、提高植物生产力以及缓解环境压力带来的挑战的创新技术的需求不断增长，这项研究至关重要。本研究聚焦于珍稀的金马仑高原白草莓植株（凤梨草莓），并解决了直接诱导芽苗过程中愈伤组织产生和玻璃化等问题。该研究旨在调查GO对白草莓植株再生过程和遗传稳定性的影响，并使用分子标记确保离体繁殖的植株保持品种纯正。为此，采用茎尖外植体，并在Murashige和Skoog（MS）培养基中添加不同浓度的GO（0、2.5、5.0、7.5、10毫克/升），持续六周。结果表明，促进白草莓幼苗发育的最佳浓度为7.5毫克/升的GO。该研究还表明，在MS培养基中添加7.5毫克/升的GO与8微摩尔的噻二唑苯基脲（TDZ）相结合，有助于诱导多芽。此外，经GO处理的离体植株的克隆保真度显示遗传相似度超过97%。这些结果证实，较低浓度的GO可改善植物发育和稳定性。因此，这种纳米材料对草莓植株的生长具有积极影响，非常适合用于草莓组织培养。
