Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, 14155-6453 Tehran, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, 14155-6453 Tehran, Iran.
Neurosci Lett. 2023 Nov 20;817:137531. doi: 10.1016/j.neulet.2023.137531. Epub 2023 Oct 18.
In several studies, the regulatory role of the neuropeptide W (NPW) system in food intake has been demonstrated. Considering the lack of avian studies in this field, the current research was conducted to evaluate the effects of intracerebroventricular (ICV) infusion of NPW and its interferences with corticotropin, melanocortin, and neuropeptide Y (NPY) receptors on meal consumption and feeding behaviors of broilers. In the first experiment, birds were injected with NPW (0.75, 1.5, and 3 nmol) in addition to saline. In the second experiment, saline, CRF1 receptor antagonist (NBI35965, 30 μg), NPW (3 nmol), and simultaneous injections of NBI35965 and NPW were performed. Experiments 3-8 were identical to experiment 2, except that CRF2 receptor antagonist (K41498, 30 μg), MC3/MC4 receptor antagonist (SHU9119, 0.5 nmol), MC4 receptor antagonist (HS024, 0.5 nmol), NPY1 receptor antagonist (BMS193885, 1.25 nmol), NPY2 receptor antagonist (CYM9484, 1.25 nmol), and NPY5 receptor (antagonist L-152,804, 1.25 nmol) were administrated instead of NBI35965. After that, cumulative feed intake and feeding behavior were monitored for 2 h and 30 min after injections, respectively. Following the infusion of NPW (1.5 and 3 nmol), there was a significant stimulation of meal consumption in chickens (P < 0.05). Concomitant injection of NBI35965 and K41498 with NPW enhanced the appetite-increasing effect of NPW (P < 0.05); while BMS193885 suppressed this effect of NPW (P < 0.05). Injection of SHU9119, HS024, CYM9484, and L-152804 with NPW at the same time, had no significant effect on NPW-induced hyperphagia (P > 0.05). NPW also significantly decreased the standing period and the number of jumps, steps, and exploratory pecks, and led to an increase in sitting period and feeding pecks (P < 0.05). Based on the observations, it seems that NPW-induced hyperphagia could be mediated through CRF1, CRF2, and NPY1 receptors in neonatal broilers.
在几项研究中，已经证明了神经肽 W（NPW）系统在摄食中的调节作用。考虑到在这个领域缺乏禽类研究，因此进行了当前的研究，以评估脑室注射 NPW 及其与促肾上腺皮质激素、黑色素浓集素和神经肽 Y（NPY）受体的相互作用对肉鸡摄食和采食行为的影响。在第一个实验中，除盐水外，鸟类还注射了 NPW（0.75、1.5 和 3 毫摩尔）。在第二个实验中，注射了盐水、CRF1 受体拮抗剂（NBI35965，30 微克）、NPW（3 毫摩尔）和同时注射 NBI35965 和 NPW。实验 3-8 与实验 2 相同，只是 CRF2 受体拮抗剂（K41498，30 微克）、MC3/MC4 受体拮抗剂（SHU9119，0.5 毫摩尔）、MC4 受体拮抗剂（HS024，0.5 毫摩尔）、NPY1 受体拮抗剂（BMS193885，1.25 毫摩尔）、NPY2 受体拮抗剂（CYM9484，1.25 毫摩尔）和 NPY5 受体（拮抗剂 L-152,804，1.25 毫摩尔）代替 NBI35965 进行了注射。之后，分别在注射后 2 小时和 30 分钟监测累积饲料摄入量和采食行为。在注射 NPW（1.5 和 3 毫摩尔）后，鸡的摄食明显增加（P<0.05）。同时注射 NBI35965 和 K41498 与 NPW 增强了 NPW 的食欲增加作用（P<0.05）；而 BMS193885 抑制了 NPW 的这种作用（P<0.05）。同时注射 SHU9119、HS024、CYM9484 和 L-152804 与 NPW 对 NPW 诱导的多食无明显影响（P>0.05）。NPW 还显著减少站立时间和跳跃、步骤和探索啄的次数，并导致坐立时间和采食啄的增加（P<0.05）。根据这些观察结果，NPW 诱导的多食可能是通过新生肉鸡中的 CRF1、CRF2 和 NPY1 受体介导的。
