Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Neurosci Lett. 2023 Jun 21;808:137283. doi: 10.1016/j.neulet.2023.137283. Epub 2023 May 2.
BACKGROUND & AIM: Adiponectin is a member of the adipokine family and contributes to regulating energy homeostasis, reproduction, and various biological functions, such as insulin receptor signaling pathway sensitivity, mitochondrial biogenesis, oxidative metabolism, neurogenesis, and suppression of inflammation. This study aimed to investigate the effects of intracerebroventricular (ICV) injection of adiponectin and its interaction with the neuropeptide Y (NPY) and GABAergic systems on central appetite regulation in neonatal layer-type chickens.
MATERIALS & METHODS: In this study, 6 experiments were conducted, each of which included 4 experimental groups. In the first experiment, the chickens were injected with saline and adiponectin (20.73, 41.45, and 62.18 nmol). In the second experiment, saline, adiponectin (62.18 nmol), B5063 (NPY1 receptor antagonist, 2.12 nmol), and simultaneous injections of adiponectin and B5063 were performed. Experiments 3 to 6 were done in the same way to experiment 1, but the chickens were injected with SF22 (NPY2 receptor antagonist, 2.66 nmol), SML0891 (NPY5 receptor antagonist, 2.89 nmol), picrotoxin (GABAA receptor antagonist, 0.89 nmol), CGP54626 (GABAB receptor antagonist, 0.047 nmol) instead of B5063. Feed consumption was measured 120 min after the injection.
A dose-dependent increase in appetite was observed after the injection of adiponectin (20.73, 41.45, and 62.18 nmol) (P < 0.05). The injection of B5063 + adiponectin attenuated the hyperphagic effect of adiponectin (P < 0.05). In addition, co-injection of picrotoxin and adiponectin significantly decreased adiponectin-induced hyperphagia (P < 0.05). In addition, adiponectin significantly increased the number of steps, jumps, exploratory food, pecks, and standing time, while decreasing sitting time and rest time (P < 0.05).
These results suggest that the hyperphagic effects of adiponectin are probably mediated through NPY1 and GABAA receptors in neonatal layer-type chickens.
脂联素是脂肪因子家族的一员，有助于调节能量稳态、生殖和各种生物学功能，如胰岛素受体信号通路敏感性、线粒体生物发生、氧化代谢、神经发生和炎症抑制。本研究旨在探讨侧脑室（ICV）注射脂联素及其与神经肽 Y（NPY）和 GABA 能系统相互作用对新生层状鸡中枢食欲调节的影响。
本研究共进行了 6 项实验，每项实验均包括 4 个实验组。在第 1 项实验中，鸡分别注射生理盐水和脂联素（20.73、41.45 和 62.18nmol）。在第 2 项实验中，鸡分别注射生理盐水、脂联素（62.18nmol）、B5063（NPY1 受体拮抗剂，2.12nmol）和同时注射脂联素和 B5063。实验 3 至 6 以与实验 1 相同的方式进行，但鸡分别注射 SF22（NPY2 受体拮抗剂，2.66nmol）、SML0891（NPY5 受体拮抗剂，2.89nmol）、戊四氮（GABAA 受体拮抗剂，0.89nmol）和 CGP54626（GABAB 受体拮抗剂，0.047nmol）代替 B5063。注射后 120 分钟测量采食量。
注射脂联素（20.73、41.45 和 62.18nmol）后，食欲呈剂量依赖性增加（P<0.05）。注射 B5063+脂联素可减弱脂联素的促食作用（P<0.05）。此外，同时注射戊四氮和脂联素可显著降低脂联素诱导的食欲亢进（P<0.05）。此外，脂联素显著增加了鸡的步数、跳跃、探食、啄食和站立时间，同时减少了鸡的坐立时间和休息时间（P<0.05）。
这些结果表明，脂联素的促食作用可能是通过新生层状鸡的 NPY1 和 GABAA 受体介导的。
