Schnur P, Raigoza V P, Sanchez M R, Kulkosky P J
Pharmacol Biochem Behav. 1986 Nov;25(5):1067-70. doi: 10.1016/0091-3057(86)90086-9.
Three experimental replications were used to test the effects of three doses (25, 50 or 75 micrograms/kg) of cholecystokinin octapeptide (CCK-8) on morphine induced changes in activity. For each dose of CCK-8, running wheel activity of golden Syrian hamsters was monitored for three hours following a series of two injections. The first injection consisted of either saline or CCK-8, the second of either saline or morphine sulfate (15 mg/kg). Thus, in each replication four groups were created: Group SAL/SAL (n = 8) received two saline injections, Group CCK/SAL (n = 8) an injection of CCK-8 followed by an injection of saline, Group SAL/MS (n = 8) an injection of saline followed by an injection of morphine and Group CCK/MS (n = 8) an injection of CCK-8 followed by an injection of morphine. Results indicated that a 25 micrograms/kg dose of CCK-8 blocked the hypoactivity elicited by morphine 40-60 min after opiate injection, whereas a 75 micrograms/kg dose of CCK-8 blocked the hyperactivity elicited by morphine 80-100 min after opiate injection. These findings are consistent with previous reports that CCK-8 antagonizes the effects of opiate agonists on a variety of behaviors and is supportive of the hypothesis that endogenous CCK-8 may antagonize endogenous opioid peptides in the control of behavior.
进行了三次实验重复，以测试三种剂量（25、50或75微克/千克）的胆囊收缩素八肽（CCK - 8）对吗啡诱导的活动变化的影响。对于每种剂量的CCK - 8，在进行一系列两次注射后的三个小时内监测金黄仓鼠的转轮活动。第一次注射为生理盐水或CCK - 8，第二次注射为生理盐水或硫酸吗啡（15毫克/千克）。因此，在每次重复实验中创建了四组：SAL/SAL组（n = 8）接受两次生理盐水注射，CCK/SAL组（n = 8）先注射CCK - 8后注射生理盐水，SAL/MS组（n = 8）先注射生理盐水后注射吗啡，CCK/MS组（n = 8）先注射CCK - 8后注射吗啡。结果表明，25微克/千克剂量的CCK - 8可在注射阿片类药物后40 - 60分钟阻断吗啡引起的活动减退，而75微克/千克剂量的CCK - 8可在注射阿片类药物后80 - 100分钟阻断吗啡引起的活动亢进。这些发现与之前关于CCK - 8拮抗阿片类激动剂对多种行为影响的报道一致，并支持内源性CCK - 8可能在行为控制中拮抗内源性阿片肽的假说。
