Eskil Vocational School, Laboratory and Veterinary Science, Aksaray University, Aksaray, Turkey.
Department of Pathology, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Turkey.
Sci Rep. 2023 Oct 20;13(1):17929. doi: 10.1038/s41598-023-44376-0.
This study investigated the cytotoxic effects of oxidative stress (OS), high mobility group box 1 (HMGB1), ADAMTS (A disintegrin and metalloproteinase with thrombospondin motifs), and neuropathology associated with coenurus cerebralis (Taenia multiceps). ADAMTS-13, HMGB1, glutathione reductase (GR), copper/zinc superoxide dismutase (Cu/Zn SOD), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) expression levels were studied. The study found that ADAMTS-13 (P < 0.005), HMGB1 (P < 0.005), GR (P < 0.005), Cu/Zn SOD (P < 0.005), and 8-OHdG (P < 0.005) levels were significantly higher in T. multiceps (c. cerebralis)-infected animals compared to healthy control animals. This study's most important finding was that HMGB1 up-regulation in neurons, endothelial cells, and glial cells can directly cause brain parenchymal destruction and that HMGB1-mediated oxidative stress plays a crucial role in the neuropathogenesis of coenurosis. The results also showed that increased levels of ADAMTS-13 may play a pivotal role in regulating and protecting the blood-brain barrier integrity and neuroprotection. These findings also suggest that ADAMTS-13 and HMGB1 compete in the prevention or formation of microthrombi, which was regarded as a remarkable finding. ADAMTS-13 and HMGB1 are valuable biomarkers for disease risk assessment, estimating host neuropathy following T. multiceps (c. cerebralis) exposure, and providing a new therapeutic target. This is the first study to show that HMGB1 and ADAMTS-13 are expressed in reactive cells and are associated with neuroimmunopathology in coenurosis.
本研究调查了氧化应激（OS）、高迁移率族蛋白 B1（HMGB1）、解整合素金属蛋白酶与凝血酶 13（ADAMTS-13）以及与多头绦虫（细粒棘球蚴）相关的神经病理学之间的细胞毒性作用。研究了 ADAMTS-13、HMGB1、谷胱甘肽还原酶（GR）、铜/锌超氧化物歧化酶（Cu/Zn SOD）和 8-羟基-2'-脱氧鸟苷（8-OHdG）的表达水平。研究发现，多头绦虫（细粒棘球蚴）感染动物的 ADAMTS-13（P<0.005）、HMGB1（P<0.005）、GR（P<0.005）、Cu/Zn SOD（P<0.005）和 8-OHdG（P<0.005）水平明显高于健康对照组动物。本研究最重要的发现是，神经元、内皮细胞和神经胶质细胞中 HMGB1 的上调可直接导致脑实质破坏，HMGB1 介导的氧化应激在多头绦虫（细粒棘球蚴）引起的神经发病机制中起关键作用。结果还表明，ADAMTS-13 水平的升高可能在调节和保护血脑屏障完整性和神经保护方面发挥关键作用。这些发现还表明，ADAMTS-13 和 HMGB1 可能在预防或形成微血栓方面存在竞争，这被认为是一个显著的发现。ADAMTS-13 和 HMGB1 是评估疾病风险、估计宿主在暴露于多头绦虫（细粒棘球蚴）后发生神经病变以及提供新的治疗靶点的有价值的生物标志物。这是第一项表明 HMGB1 和 ADAMTS-13 在反应性细胞中表达并与多头绦虫（细粒棘球蚴）相关神经免疫病理学相关的研究。
