Li Wen Hui, Qu Zi Gang, Zhang Nian Zhang, Yue Long, Gai Wen Yan, Jia Wan Zhong, Luo Jian Xun, Yin Hong, Fu Bao Quan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of the Ministry of Agriculture, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of the Ministry of Agriculture, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China ; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease, Yangzhou, China.
Iran J Parasitol. 2014 Oct-Dec;9(4):568-73.
Taenia multiceps is a cestode parasite with its larval stage (metacestode), Coenurus cerebralis, mainly encysts in the central nervous system of sheep and other livestock causing cerebralis coenurosis. Since, treatment of coenurosis with chemotherapy showed little effect and surgical removal of cysts is not advisable in field conditions, vaccination is useful to control coenurosis. Previous study indicated that immunization with T. multiceps metacestode antigens could induce protection in sheep against coenurosis, so the aim of this study was to identify T. multiceps metacestode antigens in order to find potential vaccine development candidates for further study.
The protein extracts from the larval T. multiceps were analyzed by two-dimensional electrophoresis (2-DE) and characterized by mass spectrometry.
A total of 150 protein spots were detected with isoelectric point (pI) value from 4.97 to 9.65 and molecular weight from 14 to 98 kDa. Twenty-two protein identities were determined by mass spectrometry and 15 unique proteins were obtained. Functional annotation revealed that some of these proteins are involved in catalytic activity, binding, metabolic, cellular process and stress response. Among these molecules are antioxidant proteins (peroxiredoxin and glutathione-S-transferase), glycolytic enzymes (malate dehydrogenase and enolase), proteins with chaperone activity (heat shock protein 70 and small heat shock protein), and structural proteins (actin, actin modulator protein and paramyosin).
The identification of T. multiceps metacestode protein will provide valuable information to elucidate their specific roles in the parasitism and screen new targets for vaccine development.
多头绦虫是一种绦虫寄生虫，其幼虫阶段（中绦期幼虫）脑多头蚴主要寄生于绵羊和其他家畜的中枢神经系统，引起脑多头蚴病。由于化疗对多头蚴病的治疗效果不佳，且在野外条件下手术摘除囊肿并不可取，因此疫苗接种对控制多头蚴病很有用。先前的研究表明，用多头绦虫中绦期幼虫抗原进行免疫可诱导绵羊对多头蚴病产生保护作用，因此本研究的目的是鉴定多头绦虫中绦期幼虫抗原，以寻找潜在的疫苗开发候选物进行进一步研究。
采用二维电泳（2-DE）分析多头绦虫幼虫的蛋白质提取物，并通过质谱进行表征。
共检测到150个蛋白点，等电点（pI）值为4.97至9.65，分子量为14至98 kDa。通过质谱确定了22种蛋白质的身份，获得了15种独特的蛋白质。功能注释显示，其中一些蛋白质参与催化活性、结合、代谢、细胞过程和应激反应。这些分子包括抗氧化蛋白（过氧化物酶和谷胱甘肽-S-转移酶）、糖酵解酶（苹果酸脱氢酶和烯醇酶）、具有伴侣活性的蛋白质（热休克蛋白70和小热休克蛋白）以及结构蛋白（肌动蛋白、肌动蛋白调节蛋白和副肌球蛋白）。
多头绦虫中绦期幼虫蛋白质的鉴定将为阐明其在寄生过程中的具体作用和筛选疫苗开发的新靶点提供有价值的信息。
