Laboratoire de Recherche sur les Zones Arides (LRZA), Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, 16111, Bab Ezzouar, Algiers, Algeria.
Laboratoire de Recherche sur les Zones Arides (LRZA), Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, 16111, Bab Ezzouar, Algiers, Algeria; Centre de Recherche Scientifique et Techniques sur les Régions Arides (CRSTRA), Biskra, Algeria.
J Ethnopharmacol. 2024 Jan 30;319(Pt 3):117333. doi: 10.1016/j.jep.2023.117333. Epub 2023 Oct 20.
Atriplex lindleyi Moq. subsp. inflata from Algeria is known for its traditional use for therapeutic properties.
We analysed quantitatively and qualitatively the infusion and diethyl ether, n-butanol and ethereal extracts of A. lindleyi, as well as assessed their acute toxicity, anti-inflammatory, antinociceptive and antioxidant activities.
The total phenolic and flavonoid contents were quantified. The anti-inflammatory effect was assessed using a carrageenan-induced paw oedema assay and the antinociceptive effect was evaluated using an acetic acid-induced writhing method. In addition, antioxidant activity was examined by three tests: DPPH, reducing power and phenanthroline assays.
The quantity of total phenols in the roots of A. lindleyi was larger than in the aerial parts, while the amounts of total flavonoids were larger in the aerial parts than in the roots. The HPLC profiles allowed us to identify 32 compounds belonging to the phenolic acid and flavonoid classes. Intraperitoneal administration of the infusions and phenolic extracts in mice did not cause any symptoms of toxicity or mortality. Results revealed that the aerial parts and roots of A. lindleyi had potential anti-inflammatory and antinociceptive activities and were higher than diclofenac and paracetamol, respectively. The diethyl ether extract (DEE) of aerial parts and roots showed the greatest antioxidant activity in comparison to the other tested extracts.
The phenolic composition attempted to be identified by HPLC confers this plant's pharmacological potential. The antioxidant potential may be due to active ingredients, including ascorbic acid, in the two studied parts of A. lindleyi, which is a bioactive molecule with strong antioxidant properties. Also, detecting salicylic acid, the active antinociceptive ingredient of aspirin, gallic and ferulic acids may justify this subspecies' antinociceptive and anti-inflammatory potentials.
来自阿尔及利亚的 Atriplex lindleyi Moq. 亚种膨胀被认为具有治疗特性的传统用途。
我们定量和定性分析了 A. lindleyi 的输液和乙醚、正丁醇和乙醚提取物，并评估了它们的急性毒性、抗炎、镇痛和抗氧化活性。
定量测定总酚和类黄酮含量。采用角叉菜胶诱导足肿胀试验评估抗炎作用，采用醋酸诱导扭体法评估镇痛作用。此外，还通过三种试验：DPPH、还原力和菲咯啉试验来检查抗氧化活性。
A. lindleyi 根中的总酚含量大于地上部分，而地上部分总黄酮含量大于根。HPLC 图谱允许我们鉴定属于酚酸和类黄酮类的 32 种化合物。在小鼠中腹腔内给予输液和酚类提取物不会引起任何毒性或死亡症状。结果表明，A. lindleyi 的地上部分和根具有潜在的抗炎和镇痛活性，分别高于双氯芬酸和扑热息痛。与其他测试提取物相比，地上部分和根的二乙醚提取物（DEE）显示出最大的抗氧化活性。
HPLC 尝试鉴定的酚类成分赋予了这种植物的药理学潜力。抗氧化潜力可能归因于两种研究部分的活性成分，包括抗坏血酸，这是一种具有强抗氧化特性的生物活性分子。此外，检测水杨酸，阿司匹林的有效镇痛成分，以及没食子酸和阿魏酸，可能证明了该亚种的镇痛和抗炎潜力。
