Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, China.
FEBS Open Bio. 2023 Dec;13(12):2273-2289. doi: 10.1002/2211-5463.13720. Epub 2023 Nov 7.
Eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP) is a subtype of chronic rhinosinusitis (CRS) that is associated with the nasal cavity and sinus polyps, elevated levels of eosinophils, and dysregulated immune responses to environmental triggers. The underlying cause of ECRSwNP is not well understood, and few studies have focused on the unique features of this subtype of CRS. Our study integrated proteomic and transcriptomic data with multi-omic bioinformatics analyses. We collected nasal polyps from three ECRSwNP patients and three control patients and identified 360 differentially expressed (DE) proteins, including 119 upregulated and 241 downregulated proteins. Functional analyses revealed several significant associations with ECRSwNP, including focal adhesion, hypertrophic cardiomyopathy, and extracellular matrix (ECM)-receptor interactions. Additionally, a protein-protein interaction (PPI) network revealed seven hub proteins that may play crucial roles in the development of ECRSwNP. We also compared the proteomic data with publicly available transcriptomic data and identified a total of 1077 DE genes. Pathways enriched by the DE genes involved angiogenesis, positive regulation of cell motility, and immune responses. Furthermore, we investigated immune cell infiltration and identified biomarkers associated with eosinophil and M2 macrophage infiltration using CIBERSORT and Weighted Gene Correlation Network Analysis (WGCNA). Our results provide a more complete picture of the immune-related mechanisms underlying ECRSwNP, which could contribute to the development of more precise treatment strategies for this condition.
嗜酸粒细胞性慢性鼻-鼻窦炎伴鼻息肉（ECRSwNP）是一种慢性鼻-鼻窦炎（CRS）亚型，与鼻腔和鼻窦息肉、嗜酸性粒细胞水平升高以及对环境触发因素的免疫反应失调有关。ECRSwNP 的根本原因尚不清楚，很少有研究关注这种 CRS 亚型的独特特征。我们的研究整合了蛋白质组学和转录组学数据以及多组学生物信息学分析。我们从 3 名 ECRSwNP 患者和 3 名对照患者中收集了鼻息肉，并鉴定了 360 个差异表达（DE）蛋白，包括 119 个上调和 241 个下调蛋白。功能分析揭示了与 ECRSwNP 相关的几个重要关联，包括粘着斑、肥厚型心肌病和细胞外基质（ECM）-受体相互作用。此外，蛋白质-蛋白质相互作用（PPI）网络揭示了 7 个可能在 ECRSwNP 发展中发挥关键作用的枢纽蛋白。我们还将蛋白质组学数据与公开的转录组学数据进行了比较，总共鉴定出 1077 个 DE 基因。DE 基因富集的途径涉及血管生成、细胞运动的正调节和免疫反应。此外，我们通过 CIBERSORT 和加权基因相关网络分析（WGCNA）研究了免疫细胞浸润，并鉴定了与嗜酸性粒细胞和 M2 巨噬细胞浸润相关的生物标志物。我们的研究结果提供了 ECRSwNP 免疫相关机制的更完整图景，这可能有助于为这种疾病开发更精确的治疗策略。
