Chikata Takayuki, Gatanaga Hiroyuki, Nguyen Hung The, Mizushima Daisuke, Zhang Yu, Kuse Nozomi, Oka Shinichi, Takiguchi Masafumi
Tokyo Laboratory and Division of International Collaboration Research, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto 162-0052, Japan.
AIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8655, Japan.
iScience. 2023 Sep 29;26(11):108089. doi: 10.1016/j.isci.2023.108089. eCollection 2023 Nov 17.
Although previous studies have reported HIV-1-specific T cell responses in HIV-1-exposed seronegative (HESN) individuals, there has been no detailed analysis of these T cells against HIV-1 infection. We investigated HIV-1-specific CD8 T cell responses in 200 Japanese HESN men who have sex with men (MSM). T cell responses to 143 well-characterized HIV-1 epitope peptides were analyzed by intracellular cytokine staining assay consisting of 3-week cultures of PBMCs stimulated with peptides. HLA-B∗51:01-restricted Pol TI8-specific and HLA-A∗02:06-restricted Pol SV9-specific CD8 T cells were identified in two and one individuals, respectively, whereas CD8 T cells specific for other HLA-A∗02:06-restricted or HLA-B∗51:01 epitopes were not present in these individuals. These epitope-specific T cells recognized HIV-1-infected cells. Because these two epitopes were previously reported to be protective in HIV-1-infected individuals, these protective epitope-specific T cells might suppress HIV-1 replication in HESN-MSM individuals. The present study suggests the contribution of protective epitope-specific T cells to protection against HIV-1 infection.
尽管先前的研究报道了在HIV-1暴露血清阴性（HESN）个体中存在HIV-1特异性T细胞反应，但尚未对这些T细胞针对HIV-1感染进行详细分析。我们调查了200名与男性发生性行为的日本HESN男性中的HIV-1特异性CD8 T细胞反应。通过细胞内细胞因子染色测定法分析了对143种特征明确的HIV-1表位肽的T细胞反应，该测定法包括用肽刺激PBMC进行3周培养。分别在两名和一名个体中鉴定出HLA-B∗51:01限制的Pol TI8特异性和HLA-A∗02:06限制的Pol SV9特异性CD8 T细胞，而这些个体中不存在针对其他HLA-A∗02:06限制或HLA-B∗51:01表位的CD8 T细胞。这些表位特异性T细胞识别HIV-1感染的细胞。由于这两个表位先前据报道在HIV-1感染个体中具有保护作用，这些保护性表位特异性T细胞可能会抑制HESN-MSM个体中的HIV-1复制。本研究表明保护性表位特异性T细胞对预防HIV-1感染的作用。
