College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang, Asia, China.
Institute of Mountain Resources of Guizhou Province, Guizhou Academy of Sciences, Guiyang, Asia, China.
PeerJ. 2023 Oct 18;11:e16280. doi: 10.7717/peerj.16280. eCollection 2023.
Passionflower ( Sims) is widely distributed in tropical and subtropical areas for edible, medicinal and skin care product processing, and the market demand is large. Zinc (Zn) is a necessary trace element for plant growth and development. In many countries, the content of Zn in soil is low and/or bioavailability is low. The exogenous application of Zn has become a common agronomic measure in agriculture. However, the effect of Zn on the physiological characteristics and enzyme activity of passionflower seedlings is not clear. In this study, pot experiments were conducted to analyse the effects of different concentrations of Zn (0, 200, 400, 800 mg kg) on the plant growth, photosynthetic pigments, osmotic regulators, membrane system and antioxidant enzyme system of purple passionflower ( Sims f. ) seedlings, and Pearson correlation and principal component analyses were performed. The results showed that (1) the 200 mg kg Zn treatment increased the contents of chlorophyll a (37.65%), chlorophyll b (41.22%), chlorophyll a+b (38.59%) and carotenoids (29.74%). The value of chlorophyll a/b changed little and had no effect on leaf growth. (2) The contents of proline (Pro) and malondialdehyde (MDA) in . Sims f seedlings treated with 400 mg kg Zn increased significantly by 116.84% and 42.69%, respectively. The activities of catalase (CAT) and peroxidase (POD) increased by 16.82% and 18.70%, respectively. Superoxide dismutase (SOD), leaf area (LA), leaf perimeter (LP) and leaf width (LW) decreased significantly by 47.20%, 19.75%, 8.32% and 11.97%, respectively. (3) 800 mg kg Zn significantly increased the contents of Pro (202.56%) and MDA (26.7%) and the activities of CAT (16.00%) and POD (67.00%), while the soluble sugar (SS), SOD, LA, LP and LW decreased significantly by 36.67%, 32.86%, 23.36%, 8.32% and 11.18%, respectively. (4) There was a significant positive correlation between Pro and photosynthetic pigments and between SOD and leaf growth and a significant negative correlation between POD and SS and between SOD and MDA. (5) A low concentration (200 mg kg) of Zn promoted the growth of . Sims f seedlings and allowed stress caused by high Zn concentrations to be tolerated. The results of this study can provide a reference for the application of Zn fertilizer to . Sims f. .
西番莲（ Sims）广泛分布于热带和亚热带地区，可用于食用、药用和护肤品加工，市场需求巨大。锌（Zn）是植物生长发育所必需的微量元素。在许多国家，土壤中的 Zn 含量低和/或生物可利用性低。外源施用 Zn 已成为农业中常见的农艺措施。然而，Zn 对西番莲幼苗生理特性和酶活性的影响尚不清楚。本研究通过盆栽试验，分析了不同浓度 Zn（0、200、400、800 mg kg）对紫西番莲（ Sims f.）幼苗生长、光合色素、渗透调节剂、膜系统和抗氧化酶系统的影响，并进行了 Pearson 相关性和主成分分析。结果表明：（1）200 mg kg Zn 处理提高了叶绿素 a（37.65%）、叶绿素 b（41.22%）、叶绿素 a+b（38.59%）和类胡萝卜素（29.74%）的含量。叶绿素 a/b 的值变化不大，对叶片生长没有影响。（2）400 mg kg Zn 处理的西番莲幼苗脯氨酸（Pro）和丙二醛（MDA）含量分别显著增加了 116.84%和 42.69%，CAT 和 POD 活性分别增加了 16.82%和 18.70%。SOD、叶面积（LA）、叶周长（LP）和叶宽（LW）分别显著下降了 47.20%、19.75%、8.32%和 11.97%。（3）800 mg kg Zn 显著增加了 Pro（202.56%）和 MDA（26.7%）的含量以及 CAT（16.00%）和 POD（67.00%）的活性，而可溶性糖（SS）、SOD、LA、LP 和 LW 则分别显著下降了 36.67%、32.86%、23.36%、8.32%和 11.18%。（4）Pro 与光合色素之间呈显著正相关，SOD 与叶片生长之间呈显著负相关，POD 与 SS 和 SOD 与 MDA 之间呈显著负相关。（5）低浓度（200 mg kg）的 Zn 促进了西番莲幼苗的生长，并允许高浓度 Zn 引起的胁迫得到耐受。本研究结果可为西番莲的 Zn 肥应用提供参考。
