Wang Junrong, Qin Shun, Fan Ruidong, Peng Qiang, Hu Xiaojing, Yang Liu, Liu Zengliang, Baccelli Ivan, Migheli Quirico, Berg Gabriele, Chen Xiaoyulong, Cernava Tomislav
College of Agriculture, College of Tobacco Science, Guizhou University, Guiyang 550025, China.
International Jointed Institute of Plant Microbial Ecology and Resource Management in Guizhou University, Ministry of Agriculture, China Association of Agricultural Science Societies, Guizhou University, Guiyang 550025, China.
J Fungi (Basel). 2023 Jan 18;9(2):132. doi: 10.3390/jof9020132.
Passion fruit ( Sims) is widely cultivated in tropic and sub-tropic regions for the production of fruit, flowers, cosmetics, and for pharmacological applications. Its high economic, nutritional, and medical values elicit the market demand, and the growing areas are rapidly increasing. Leaf blight caused by is a new and emerging disease of passion fruit in Guizhou, in southwest China, where the unique karst mountainous landscape and climate conditions are considered potential areas of expansion for passion fruit production. species are the most common biocontrol and plant-growth-promotion bacteria (PGPB) resources in agricultural systems. However, little is known about the endophytic existence of s spp. in the passion fruit phyllosphere as well as their potential as biocontrol agents and PGPB. In this study, 44 endophytic strains were isolated from 15 healthy passion fruit leaves, obtained from Guangxi province, China. Through purification and molecular identification, 42 of the isolates were ascribed to species. Their inhibitory activity against was tested in vitro. Eleven endophytic spp. strains inhibited the pathogen by >65%. All of them produced biocontrol- and plant-growth-promotion-related metabolites, including indole-3-acetic acid (IAA), protease, cellulase, phosphatase, and solubilized phosphate. Furthermore, the plant growth promotion traits of the above 11 endophytic strains were tested on passion fruit seedlings. One isolate, coded GUCC4, significantly increased passion fruit stem diameter, plant height, leaf length, leaf surface, fresh weight, and dry weight. In addition, GUCC4 reduced the proline content, which indicated its potential to positively regulate passion fruit biochemical properties and resulted in plant growth promotion effects. Finally, the biocontrol efficiencies of GUCC4 against were determined in vivo under greenhouse conditions. Similarly to the fungicide mancozeb and to a commercial -based biofungicide, GUCC4 significantly reduced disease severity. These results suggest that GUCC4 has great potential as a biological control agent and as PGPB on passion fruit.
西番莲（西番莲属）在热带和亚热带地区广泛种植，用于生产水果、花卉、化妆品及用于药理学应用。其较高的经济、营养和医学价值引发了市场需求，种植面积正在迅速增加。由[病原体名称缺失]引起的叶斑病是中国西南部贵州省西番莲的一种新出现的病害，该地区独特的喀斯特山地景观和气候条件被认为是西番莲生产潜在的扩张区域。[细菌名称缺失]物种是农业系统中最常见的生物防治和植物生长促进细菌（PGPB）资源。然而，关于[细菌名称缺失]物种在西番莲叶际的内生存在情况以及它们作为生物防治剂和PGPB的潜力知之甚少。在本研究中，从中国广西省采集的15片健康西番莲叶片中分离出44株内生菌株。通过纯化和分子鉴定，其中42株分离物被归为[细菌名称缺失]物种。对它们对[病原体名称缺失]的抑制活性进行了体外测试。11株内生[细菌名称缺失]物种菌株对病原体的抑制率>65%。它们都产生了与生物防治和植物生长促进相关的代谢产物，包括吲哚 - 3 - 乙酸（IAA）、蛋白酶、纤维素酶、磷酸酶和溶解的磷酸盐。此外，对上述11株内生[细菌名称缺失]菌株的植物生长促进特性在西番莲幼苗上进行了测试。一株编号为GUCC4的分离物显著增加了西番莲的茎直径、株高、叶长、叶面积、鲜重和干重。此外，GUCC4降低了脯氨酸含量，这表明其具有积极调节西番莲生化特性并产生植物生长促进作用的潜力。最后，在温室条件下在体内测定了GUCC4对[病原体名称缺失]的生物防治效率。与杀菌剂代森锰锌和一种基于[细菌名称缺失]的商业生物杀菌剂类似，GUCC4显著降低了病害严重程度。这些结果表明，GUCC4作为西番莲的生物防治剂和PGPB具有巨大潜力。
