Thijssen Marijn, Devos Timothy, Meyfroidt Geert, Van Ranst Marc, Pourkarim Mahmoud Reza
KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, Laboratory for Clinical and Epidemiological Virology, Leuven, Belgium.
University Hospitals Leuven, Department of Haematology, Department of Microbiology and Immunology, Laboratory of Molecular Immunology (Rega Institute), KU Leuven, Leuven, Belgium.
IJID Reg. 2023 Sep 24;9:49-54. doi: 10.1016/j.ijregi.2023.09.005. eCollection 2023 Dec.
Anelloviruses have been linked with host-immunocompetence and inflammation. Here, we studied the anellovirus load in hospitalized COVID-19 patients.
We collected samples of patients recruited in the DAWN-Plasma trial that received convalescent plasma (CP) therapy (four plasma units) combined with standard of care (SOC) or SOC of alone. Plasma samples were collected on day 0 and 6 of hospitalization and we quantified anellovirus load. With multivariate models, clinical variables were associated with changes in anellovirus load.
Samples were collected on day 0 and 6 of 150 patients (103 CP + SOC and 47 SOC). Anellovirus load was higher on day 0 compared to day 6 and we found a significant drop in SOC patients. Patients receiving immunosuppressive drug had a lower anellovirus load (coefficient: 1.021, 95% confidence interval [CI] 0.270-1.772, = 0.008), while patients admitted to the emergency room displayed a higher abundance on day 0 (1.308, 95% CI 0.443-2.173, = 0.003). Unspecific markers of inflammation and organ damage, D-dimer (0.001, 95% CI <0.001-0.001, = 0.001) and lactate dehydrogenase (0.002, 95% CI 0.001-0.004, = 0.044), were positively associated with anellovirus load. Finally, anellovirus load on day 0 (-39.9, 95% CI -75.72 to -4.27, = 0.029) was negatively associated with SARS-CoV-2 antibody response on day.
The results showed associations between clinical variables and anellovirus load in COVID-19 patients. Many variables share properties related to host immunocompetence or inflammation. Therefore, we expect that anellovirus abundance displays the net state of immune activation.
环曲病毒与宿主免疫能力和炎症有关。在此，我们研究了住院的新冠肺炎患者体内的环曲病毒载量。
我们收集了参与DAWN血浆试验的患者样本，这些患者接受了恢复期血浆（CP）治疗（四个血浆单位）并联合标准治疗（SOC）或仅接受标准治疗。在住院第0天和第6天采集血浆样本，我们对环曲病毒载量进行了定量分析。通过多变量模型，将临床变量与环曲病毒载量的变化相关联。
在150名患者（103名接受CP+SOC治疗和47名接受SOC治疗）的第0天和第6天采集了样本。与第6天相比，第0天的环曲病毒载量更高，并且我们发现接受标准治疗的患者中有显著下降。接受免疫抑制药物治疗的患者环曲病毒载量较低（系数：1.021，95%置信区间[CI]0.270 - 1.772，P = 0.008），而入住急诊室的患者在第0天显示出更高的丰度（1.308，95% CI 0.443 - 2.173，P = 0.003）。炎症和器官损伤的非特异性标志物，D - 二聚体（0.001，95% CI <0.001 - 0.001，P = 0.001）和乳酸脱氢酶（0.002，95% CI 0.001 - 0.004，P = 0.044）与环曲病毒载量呈正相关。最后，第0天的环曲病毒载量（-39.9，95% CI -75.72至-4.27，P = 0.029）与当天的新冠病毒抗体反应呈负相关。
结果显示了新冠肺炎患者临床变量与环曲病毒载量之间的关联。许多变量具有与宿主免疫能力或炎症相关的特性。因此，我们预计环曲病毒丰度显示免疫激活的净状态。
