Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Medizinische Fakultät der Otto-von-Guericke Universität Magdeburg, Magdeburg, Germany.
Universitätskinderklinik, Medizinische Fakultät der Otto-von-Guericke Universität Magdeburg, Magdeburg, Germany.
Front Cell Infect Microbiol. 2023 Oct 6;13:1277522. doi: 10.3389/fcimb.2023.1277522. eCollection 2023.
(heterotypic synonym: "") is a facultative anaerobic, newly described Gram-negative rod and belongs to the Yersineacea family. Here, we report the case of a 19-month-old female infant patient who presented to the emergency unit with somnolence and fever. was isolated from a positive blood culture, taken via an implanted Broviac catheter, proving a bloodstream infection by the pathogen. The objective of this study was to utilize whole genome sequencing to assess the genes encoding potential virulence associated factors, which may play a role in host tropism, tissue invasion and the subsequent stages in the pathogenesis of a bloodstream infection with . The genome of the isolate was completely sequenced employing Illumina MiSeq and Nanopore MinION sequencing and the presumptive virulence associated factors and antimicrobial resistance genes were investigated in more detail. Additionally, we performed metabolic profiling and susceptibility testing by microdilution. The presence of predicted TcfC-like α-Pili suggests that is highly adapted to humans as a host. It utilizes flagellar and type IV pili-mediated motility, as well as a number of γ-pili and a σ-pilus, which may be used to facilitate biofilm formation and adherence to host epithelia. Additionally, long polar fimbriae may aid in tissue invasion. The bacterium possesses antioxidant factors, which may enable temporary survival in phagolysosomes, and a capsule that potentially provides protection from phagocytosis. It may acquire iron ions from erythrocytes through the type 6 secretion system and hemolysins. Furthermore, the isolate exhibits beta-lactamase-mediated penicillin and aminopenicillin resistance. Based on the analysis of the whole genome, we conclude that possesses virulence factors associated with tissue invasion and may thus be a potential opportunistic pathogen of bloodstream infections.
（异名：“”）是一种兼性厌氧、新描述的革兰氏阴性杆菌，属于耶尔森氏菌科。在这里，我们报告了一例 19 个月大的女性婴儿患者，因嗜睡和发热到急诊就诊。从植入的 Broviac 导管获得的阳性血培养中分离出 ，证实了病原体引起的血流感染。本研究的目的是利用全基因组测序评估编码潜在毒力相关因子的基因，这些因子可能在宿主嗜性、组织侵袭以及随后的血流感染发病机制阶段发挥作用。采用 Illumina MiSeq 和 Nanopore MinION 测序对分离株的基因组进行了全序列测序，并更详细地研究了推定的毒力相关因子和抗微生物药物耐药基因。此外，我们还通过微量稀释法进行了代谢谱分析和药敏试验。预测的 TcfC 样α-菌毛的存在表明 高度适应人类作为宿主。它利用鞭毛和 IV 型菌毛介导的运动，以及许多γ-菌毛和σ菌毛，这可能有助于生物膜形成和与宿主上皮的黏附。此外，长极性菌毛可能有助于组织侵袭。该细菌具有抗氧化因子，这可能使其能够在吞噬体中暂时存活，并且具有囊泡，可能提供对吞噬作用的保护。它可能通过 VI 型分泌系统和溶血素从红细胞中获取铁离子。此外，该分离株表现出β-内酰胺酶介导的青霉素和氨青霉素耐药性。基于全基因组分析，我们得出结论， 具有与组织侵袭相关的毒力因子，因此可能是血流感染的潜在机会性病原体。
