Present address: Utah Public Health Laboratory, Taylorsville, Utah, USA.
Department of Pathology, University of Utah, Salt Lake City, Utah, USA.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2703-2712. doi: 10.1099/ijsem.0.004094. Epub 2020 Mar 6.
Eight genetically related, Gram-negative bacterial strains, isolated from clinical specimens between 2012 and 2016, were submitted to arup Laboratories for species identification. The lack of species- or genus-level matches in curated 16S rRNA gene databases prompted us to undertake the polyphasic characterization of these so far undescribed organisms. Six isolates available for additional testing were oxidase negative, catalase positive, pleomorphic, Gram-negative rods displaying temperature-dependent motility and producing yellow-pigmented colonies with three distinct morphotypes: medium-sized shiny, large mucoid and agar-pitting. Biochemical reactions and sugar fermentation patterns were most similar to members of the genus . Fatty acid profiles were highly similar across all six organisms, with the major components being: C; C cyclo; C 3-OH/iso-C I; C ω7; and C ω7/C ω6. Whole-genome comparisons and multi locus sequence analysis (using the coding genes , , and ) suggest that the strains here described constitute three individual species within a novel genus related to the family . We propose for this novel taxon the name gen. nov., referring to the presentation of multiple characteristics typical of distinct genera within a single organism. Four isolates are representative of a single species: sp. nov (2016-Iso1, 2016-Iso2, type strain 2016-Iso3=DSM 110101=ATCC TSD-180 and 2013-Iso5). The remaining two isolates constitute the novel species sp. nov. (type strain 2016-Iso4=DSM 110102=ATCC TSD-182) and sp. nov. (type strain 2015-Iso6=DSM 110100=ATCC TSD-181). Our work provides the first formal characterization of the genus and forms the basis to study its taxonomic diversity.
从 2012 年至 2016 年期间，从临床标本中分离出的 8 株遗传相关的革兰氏阴性细菌菌株被提交至 arup 实验室进行种属鉴定。在经过精心整理的 16S rRNA 基因数据库中，这些菌株既没有与已知种属相匹配，也没有与任何已知属种相匹配，这促使我们对这些尚未被描述的菌株进行多相特征分析。有 6 株可供进一步测试的分离株为氧化酶阴性、触酶阳性、多形性、革兰氏阴性杆菌，具有温度依赖性运动，并产生三种不同形态的黄色色素菌落：中大型有光泽、大型黏液型和琼脂坑洼型。生化反应和糖发酵模式与属最为相似。所有 6 株菌的脂肪酸图谱高度相似，主要成分为：C；C 环；C 3-OH/iso-C I；C ω7；和 C ω7/C ω6。全基因组比较和多位点序列分析（使用编码基因、、和）表明，这里描述的菌株构成了一个新属的 3 个独立种，该新属与家族相关。我们建议将该新分类群命名为 属，nov. gen.，指的是在单个生物体中呈现出多种典型的不同 属特征。其中 4 个分离株代表了一个单一的种： sp. nov.（2016-Iso1、2016-Iso2、模式株 2016-Iso3=DSM 110101=ATCC TSD-180 和 2013-Iso5）。剩下的 2 个分离株构成了新种 sp. nov.（模式株 2016-Iso4=DSM 110102=ATCC TSD-182）和 sp. nov.（模式株 2015-Iso6=DSM 110100=ATCC TSD-181）。我们的工作首次对 属进行了正式的特征描述，并为研究其分类多样性奠定了基础。
