Kumar Naresh, Sharma Vikas, Kaur Gurpreet, Lata Charu, Dasila Hemant, Perveen Kahkashan, Khan Faheema, Gupta Vijay K, Khanam Mehrun Nisha
Department of Chemistry and Biochemistry, Eternal University, Rajgarh, India.
Department of Biochemistry, Kurukshetra University, Kurukshetra, India.
Front Microbiol. 2023 Oct 5;14:1259103. doi: 10.3389/fmicb.2023.1259103. eCollection 2023.
The escalation of harmful pollutants, including heavy metals, due to industrialization and urbanization has become a global concern. To mitigate the negative impacts of heavy metal stress on germination and early plant development, growth regulators have been employed. This study aimed to evaluate the response of mung bean () to zinc stress in the presence of brassinosteroids, focusing on seedling growth and antioxidant potential. Mung bean seedlings were treated with three concentrations of 24-epibrassinolide (EBL) (0.1, 0.2, and 0.4 PPM) with or without zinc. Results demonstrated that the application of brassinosteroids, combined with zinc stress, significantly enhanced germination percentage (about 47.06, 63.64, and 120%), speed of germination (about 39.13, 50, and 100%), seedling growth (about 38% in case of treatment combined 0.4 PPM 24-EBL and 1.5 mM ZnSO) and seedling vigor index (204% in case of treatment combined 0.4 PPM 24-EBL and 1.5 mM ZnSO) compared to zinc-treated seedlings alone after 24 h. The activities of antioxidative enzymes (catalase, ascorbate peroxidase, polyphenol oxidase, and peroxidase) and total soluble protein content decreased, while lipid peroxidation and proline content exhibited a significant increase ( ≤ 0.05) when compared to the control. However, the negative effects induced by heavy metal stress on these parameters were significantly mitigated by EBL application. Notably, the most effective concentration of EBL in overcoming zinc stress was found to be 0.4 PPM. These findings underscore the potential of exogenously applied brassinosteroids as a valuable tool in phytoremediation projects by ameliorating heavy metal stress.
由于工业化和城市化，包括重金属在内的有害污染物不断增加，已成为全球关注的问题。为了减轻重金属胁迫对种子萌发和植物早期发育的负面影响，人们采用了生长调节剂。本研究旨在评估在存在油菜素内酯的情况下绿豆（）对锌胁迫的反应，重点关注幼苗生长和抗氧化潜力。绿豆幼苗用三种浓度的24-表油菜素内酯（EBL）（0.1、0.2和0.4 ppm）处理，有无锌处理。结果表明，与单独锌处理的幼苗相比，油菜素内酯与锌胁迫联合应用显著提高了发芽率（约47.06%、63.64%和120%）、发芽速度（约39.13%、50%和100%）、幼苗生长（在0.4 ppm 24-EBL和1.5 mM ZnSO联合处理的情况下约为38%）和幼苗活力指数（在0.4 ppm 24-EBL和1.5 mM ZnSO联合处理的情况下为204%）。与对照相比，抗氧化酶（过氧化氢酶、抗坏血酸过氧化物酶、多酚氧化酶和过氧化物酶）的活性和总可溶性蛋白含量降低，而脂质过氧化和脯氨酸含量显著增加（≤0.05）。然而，EBL的应用显著减轻了重金属胁迫对这些参数的负面影响。值得注意的是，发现克服锌胁迫最有效的EBL浓度为0.4 ppm。这些发现强调了外源施用油菜素内酯作为一种有价值的工具，通过改善重金属胁迫在植物修复项目中的潜力。
