Aleem Abdul, Aslam Bilal, Alim Muhammad Bilal, Hussain Asif, Faisal Muhammad Naeem, Sindhu Zia-Ud-Din
Institute of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan.
Faculty of Medical and Health Sciences, University of Sargodha, Sargodha, Pakistan.
Pak J Pharm Sci. 2023 Sep;36(5):1425-1434.
Stellaria media L. has traditionally been used to treat inflammatory and gastrointestinal ailments. This study aimed to phytochemically characterize the S. media extract and explore its anti-ulcer efficacy against piroxicam-induced stomach lesions in Wistar rats. Phytochemical analysis was performed and antioxidant capacity of extract was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. In vivo, piroxicam (30mg/kg) was administered to induce gastric ulceration. Gastro protective effect of S. media extract was observed at 150, 300 and 450mg/kg, respectively. While omeprazole (20mg/kg) was used as a conventional anti-ulcer drug. After oral treatment for 14 days, stomach acidic secretions, ulcerogenic indices, hematological markers and oxidative stress parameters were assessed along with histological examination. The existence of polyphenol contents in S. media extract was confirmed in correlation to a marked DPPH inhibition (IC 27.94µg/mL). S. media extract resulted in a dose-dependent elevation in gastric pH while a decrease in acid volume, acidity and ulceration. Also, S. media extract administration restored the impaired hematological markers (RBCs, Hb, WBCs and PLTs) and decreased oxidative stress by reducing oxidants (TOS and MDA) while raising antioxidants (TAC and CAT). Furthermore, gastric histological results corroborated the aforementioned findings. Conclusively, S. media could provide a promising protective effect against drug-induced gastric ulceration.
繁缕传统上被用于治疗炎症和胃肠道疾病。本研究旨在对繁缕提取物进行植物化学特征分析，并探讨其对吡罗昔康诱导的Wistar大鼠胃损伤的抗溃疡功效。进行了植物化学分析，并使用1,1-二苯基-2-苦基肼（DPPH）法测定提取物的抗氧化能力。在体内，给予吡罗昔康（30mg/kg）以诱导胃溃疡。分别在150、300和450mg/kg剂量下观察到繁缕提取物的胃保护作用。同时，奥美拉唑（20mg/kg）用作传统抗溃疡药物。口服治疗14天后，评估胃酸分泌、溃疡指数、血液学指标和氧化应激参数，并进行组织学检查。证实繁缕提取物中存在多酚成分，这与显著的DPPH抑制作用（IC 27.94µg/mL）相关。繁缕提取物导致胃pH值呈剂量依赖性升高，同时胃酸体积、酸度和溃疡程度降低。此外，给予繁缕提取物可恢复受损的血液学指标（红细胞、血红蛋白、白细胞和血小板），并通过减少氧化剂（总氧化应激和丙二醛）同时增加抗氧化剂（总抗氧化能力和过氧化氢酶）来降低氧化应激。此外，胃组织学结果证实了上述发现。总之，繁缕对药物诱导的胃溃疡可能具有良好的保护作用。
