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细叶百里香的化学表征、杀线虫活性和抗氧化活性

Chemical characterization, nematicidal and antioxidant activities of Thymus linearis Benth.

作者信息

Saleem Rubeena, Sana Aisha, Ghani Yusra, Mushtaq Faiza, Abid Rubina, Alam Jan, Tariq Marium, Dawar Shahnaz, Sultana Razia, Faizi Shaheen

机构信息

Department of Chemistry, Jinnah University for Women, Karachi, Pakistan/International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.

Department of Pharmaceutical Chemistry, Jinnah University for Women, Karachi, Pakistan.

出版信息

Pak J Pharm Sci. 2023 Sep;36(5):1561-1571.

PMID:37869932
Abstract

Thymus linearis and its essential oil (EO) are used to cure a range of diseases in traditional medicine. GC-MS analysis of Thymus linearis EO revealed the presence of sixty-four components. Thymol (50.62%), carvacrol (13.23 %), carvacrol acetate (7.72%), -bisabolene (5.47%) and o-cymene (5.47%) are the only five basic constituents in the oil which accounts for 82.07% of oil. When compared to other compounds, the EO and its primary components thymol and carvacrol had the largest proportion of mortality in Meloidogyne javanica. Carvacrol has also been proven to be helpful in suppressing the hatching of M. javanica eggs. This is the first time T. linearis EO and its constituents, such as borneol and caryophyllene, have been studied for nematicidal action. The antioxidant activity of EO components and active compounds was assessed using the ABTS radical scavenging method. Thymol and carvacrol were found to exhibit high antioxidant activity. The IC of thymol and carvacrol are found to be 38.18 g/ml and 49.65 g/ml, respectively and are comparable to the positive control trolox (47.12 g/ml). Results clearly showed high potency for EO and its constituents, thymol and carvacrol as nematicidal and antioxidant agents.

摘要

线状百里香及其精油(EO)在传统医学中用于治疗一系列疾病。对线状百里香精油的气相色谱 - 质谱分析显示存在64种成分。百里香酚(50.62%)、香芹酚(13.23%)、乙酸香芹酯(7.72%)、β-红没药烯(5.47%)和邻伞花烃(5.47%)是该精油仅有的五种主要成分,占精油的82.07%。与其他化合物相比,该精油及其主要成分百里香酚和香芹酚对爪哇根结线虫的致死率最高。香芹酚也已被证明有助于抑制爪哇根结线虫卵的孵化。这是首次对线状百里香精油及其成分如冰片和石竹烯的杀线虫作用进行研究。使用ABTS自由基清除法评估了精油成分和活性化合物的抗氧化活性。发现百里香酚和香芹酚具有高抗氧化活性。百里香酚和香芹酚的半数抑制浓度(IC)分别为38.18微克/毫升和49.65微克/毫升,与阳性对照曲克芦丁(47.12微克/毫升)相当。结果清楚地表明,该精油及其成分百里香酚和香芹酚作为杀线虫剂和抗氧化剂具有高效能。

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