Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
ACS Sens. 2023 Nov 24;8(11):3988-4007. doi: 10.1021/acssensors.3c01338. Epub 2023 Oct 23.
Point-of-care (POC) detection is getting more and more attention in many fields due to its accuracy and on-site test property. The CRISPR/Cas12a system is endowed with excellent sensitivity, target identification specificity, and signal amplification ability in biosensing because of its unique trans-cleavage ability. As a result, a lot of research has been made to develop CRISPR/Cas12a-based biosensors. In this review, we focused on signal readout strategies and summarized recent sensitivity-improving strategies in fluorescence, colorimetric, and electrochemical signaling. Then we introduced novel portability-improving strategies based on lateral flow assays (LFAs), microfluidic chips, simplified instruments, and one-pot design. In the end, we also provide our outlook for the future development of CRISPR/Cas12a biosensors.
即时检测（POC）因其准确性和现场测试特性，在许多领域受到越来越多的关注。CRISPR/Cas12a 系统具有独特的转切割能力，在生物传感中赋予了优异的灵敏度、靶标识别特异性和信号放大能力。因此，已经进行了大量的研究来开发基于 CRISPR/Cas12a 的生物传感器。在这篇综述中，我们重点介绍了信号读出策略，并总结了最近在荧光、比色和电化学信号方面提高灵敏度的策略。然后，我们介绍了基于侧向流动分析（LFA）、微流控芯片、简化仪器和一锅设计的新型便携性改进策略。最后，我们还对 CRISPR/Cas12a 生物传感器的未来发展提出了展望。
