Ondokuz Mayıs University, Faculty of Science, Department of Biology, Samsun, 55139, Türkiye.
University of Usak, Faculty of Agriculture, Departman of Horticulture, Uşak, 64200, Türkiye.
Chem Biodivers. 2023 Dec;20(12):e202301218. doi: 10.1002/cbdv.202301218. Epub 2023 Nov 13.
This study aims to investigate the functional groups and phytochemical profile of Anacamptis coriophora seeds, tubers, and flowers. Symbiotic seedlings produced using the ex vitro method were transferred to their natural habitat and grown to analyze the functional groups and phytochemical profiles of tubers and flowers. The life cycles of the transferred seedlings were monitored, and tubers and flowers were harvested for analysis. ATR-FTIR spectroscopy revealed the presence of functional groups such as polysaccharides, lignin, and proteins in both tubers and flowers. Differences in spectral frequencies between first-year and second-year tubers were observed. Fatty acid analysis identified 30 different compounds in seeds, flowers, and tubers, with linoleic acid being the most abundant (27 % in seeds, 33 % in tubers), and palmitic acid present in flowers (24 %). GC-MS analysis of ethanol extracts from these components highlighted the presence of 32 compounds, including hydroxyacetic acid, hydrazide, cytidine (Z)-7-hexadecenal, 2,2-dimethoxyethane, 2,5,6-trimethyldecane, and butanamide, 4-amino-N-hydroxy. A. coriophora's tubers, flowers, and seeds may contain active metabolites with therapeutic potential. These results are valuable for the commercial cultivation of the plant.
本研究旨在探究角盘兰种子、块茎和花朵的官能团和植物化学成分。使用离体方法生产的共生苗被转移到其自然栖息地进行生长，以分析块茎和花朵的官能团和植物化学成分。监测转移苗的生命周期，并收获块茎和花朵进行分析。ATR-FTIR 光谱揭示了多糖、木质素和蛋白质等官能团存在于块茎和花朵中。观察到一年生和二年生块茎之间的光谱频率差异。脂肪酸分析鉴定了种子、花朵和块茎中的 30 种不同化合物，其中亚油酸最为丰富（种子中 27%，块茎中 33%），棕榈酸存在于花朵中（24%）。对这些成分的乙醇提取物进行 GC-MS 分析，突出显示了 32 种化合物的存在，包括羟基乙酸、酰肼、胞嘧啶（Z）-7-十六烯醛、2,2-二甲氧基乙烷、2,5,6-三甲基癸烷和丁酰胺，4-氨基-N-羟基。角盘兰的块茎、花朵和种子可能含有具有治疗潜力的活性代谢物。这些结果对该植物的商业种植具有重要意义。
