下调卵巢子宫内膜异位症不孕患者卵泡液中的基因。

Down-regulation of the gene in follicular fluid of infertile women with ovarian endometriosis.

机构信息

The First Hospital of Lanzhou University, Lanzhou, China.

Gansu Key Laboratory for Reproductive Medicine and Embryology, Lanzhou, China.

出版信息

Gynecol Endocrinol. 2023 Dec;39(1):2269273. doi: 10.1080/09513590.2023.2269273. Epub 2023 Oct 23.

DOI:10.1080/09513590.2023.2269273

PMID:37871609

Abstract

OBJECTIVE

To evaluate FTO concentrations in follicular fluid (FF) of women with ovarian endometriosis (OE) and controls women without OE undergoing fertilization-embryo transfer (IVF-ET).

METHODS

FTO concentrations in FF were measured in 74 patients (37 in the control group and 37 in the OE group) by ELISA. We measured the expression of FTO in GCs of 40 patients (19 in the control group and 21 in the OE group) by RT-qPCR. The level of mA in GCs was measured in 20 patients (10 in the control group and 10 in the OE group) by colorimetry.

RESULTS

Compared with the control group, FTO concentrations in FF (6.92 ± 0.44 5.67 ± 0.40 ng/ml) ( <.05) and FTO mRNA level in GCs of OE group were decreased significantly ( <.05), and the level of mA was increased (0.21 ± 0.01 0.17 ± 0.03 ng) ( >.05).

CONCLUSIONS

The FTO concentrations in FF of infertility women with OE are decreased, which may be related to the impaired oocyte quality in endometriosis patients.

摘要

目的

评估卵巢子宫内膜异位症（OE）患者和接受体外受精-胚胎移植（IVF-ET）的无 OE 对照组女性卵泡液（FF）中的 FTO 浓度。

方法

通过 ELISA 测量 74 名患者（对照组 37 名，OE 组 37 名）FF 中的 FTO 浓度。通过 RT-qPCR 测量 40 名患者（对照组 19 名，OE 组 21 名）GC 中 FTO 的表达。通过比色法测量 20 名患者（对照组 10 名，OE 组 10 名）GC 中的 mA 水平。