Gluud C, Bennett P
Scand J Clin Lab Invest. 1986 Nov;46(7):647-53. doi: 10.3109/00365518609083727.
The serum concentrations of testosterone and of non-protein bound testosterone were determined in 28 men with alcoholic liver disease having normal to decreased serum albumin concentrations and normal to raised SHBG concentrations. Serum testosterone concentrations determined with two radioimmunoassays using different purification procedures and antibody batches did not differ significantly and correlated significantly (r=0.91; p less than 0.001). The median serum concentration of non-protein bound testosterone was 0.265 nmol/l (range 0.068-0.495 nmol/l) when determined by equilibrium dialysis and 0.232 nmol/l (range 0.042-0.610 nmol/l) when calculated according to the law of mass action. This difference is insignificant. The concentrations of non-protein bound testosterone determined by the two methods correlated significantly (r=0.83; p less than 0.001). In the calculation of non-protein bound testosterone, the actual serum albumin concentration can be replaced by a fixed albumin concentration (r=0.99; p less than 0.001). Further, in these patients the serum concentration of non-protein bound testosterone can be expressed by the testosterone/SHBG ratio (r=0.97; p less than 0.001).
对28名患有酒精性肝病的男性进行了血清睾酮和非蛋白结合睾酮浓度的测定，这些男性的血清白蛋白浓度正常至降低，性激素结合球蛋白（SHBG）浓度正常至升高。使用不同纯化程序和抗体批次的两种放射免疫测定法测定的血清睾酮浓度无显著差异，且相关性显著（r = 0.91；p＜0.001）。通过平衡透析测定时，非蛋白结合睾酮的血清中位数浓度为0.265 nmol/L（范围0.068 - 0.495 nmol/L），根据质量作用定律计算时为0.232 nmol/L（范围0.042 - 0.610 nmol/L）。这种差异不显著。两种方法测定的非蛋白结合睾酮浓度相关性显著（r = 0.83；p＜0.001）。在计算非蛋白结合睾酮时，实际血清白蛋白浓度可用固定白蛋白浓度替代（r = 0.99；p＜0.001）。此外，在这些患者中，非蛋白结合睾酮的血清浓度可用睾酮/SHBG比值表示（r = 0.97；p＜0.001）。
