Hetland G, Johnson E, Aasebø U
Scand J Immunol. 1986 Nov;24(5):603-8. doi: 10.1111/j.1365-3083.1986.tb02176.x.
Attachment of protein to agarose beads cultured with macrophages in protein-free medium containing 3H-leucine, shows that de novo synthesis of protein with affinity to the beads takes place. We also found that monoclonal antibodies against human C3c, C3g, and a C9-neoantigen as well as polyclonal antibodies against human C5 and C9, bound to agarose beads that had been kept with the macrophage cultures. Demonstration of C3 derivatives on the agarose beads shows that the essential complement factors of the alternative pathway are synthesized and have been activated by the beads. Deposition of C5 and the detection of a neoantigen of C9 on the beads, indicates that the whole terminal complement pathway has been formed and activated. We conclude that human alveolar macrophages form in vitro the functional alternative pathway of complement, C5 and C9, and we have indirect evidence for synthesis of C6, C7, and C8.
在含有3H-亮氨酸的无蛋白培养基中培养的巨噬细胞与琼脂糖珠结合的蛋白质表明，发生了对珠子具有亲和力的蛋白质的从头合成。我们还发现，针对人C3c、C3g和C9新抗原的单克隆抗体以及针对人C5和C9的多克隆抗体，与与巨噬细胞培养物一起保存的琼脂糖珠结合。琼脂糖珠上C3衍生物的证明表明，替代途径的基本补体因子已被合成并被珠子激活。C5在珠子上的沉积以及C9新抗原的检测表明，整个末端补体途径已形成并被激活。我们得出结论，人肺泡巨噬细胞在体外形成补体C5和C9的功能性替代途径，并且我们有C6、C7和C8合成的间接证据。
