Hetland G, Johnson E, Falk R J, Eskeland T
Scand J Immunol. 1986 Oct;24(4):421-8. doi: 10.1111/j.1365-3083.1986.tb02130.x.
Monocytes cultured under serum-free conditions secreted protein which bound covalently and non-covalently to agarose beads, an activator of the alternative pathway of complement. There was a significantly binding of monoclonal anti-C3c antibodies, polyclonal anti-C5, anti-C6, anti-C7, anti-C8, and anti-C9 antibodies, and of a monoclonal antibody against a neoantigen of polymerized C9 to agarose beads incubated with the monocytes for 24, 48, 72 or 96 h. From these results, we conclude that monocytes produce C5, C6, C7, C8 and C9 that assemble as the terminal complement complex on the surface of the agarose beads. Activation by agarose of the alternative pathway with generation of particle bound C3 and C5 convertases is a prerequisite for the subsequent formation of the terminal complement complex. Whether SC5b-9 or the membrane attack of complement (C5b-9) is formed on the beads will be examined.
在无血清条件下培养的单核细胞分泌的蛋白质与琼脂糖珠共价结合和非共价结合，琼脂糖珠是补体替代途径的激活剂。与单核细胞一起孵育24、48、72或96小时的琼脂糖珠与单克隆抗C3c抗体、多克隆抗C5、抗C6、抗C7、抗C8和抗C9抗体以及针对聚合C9新抗原的单克隆抗体有显著结合。从这些结果，我们得出结论，单核细胞产生C5、C6、C7、C8和C9，它们在琼脂糖珠表面组装成末端补体复合物。琼脂糖激活替代途径并产生颗粒结合的C3和C5转化酶是随后形成末端补体复合物的先决条件。将研究是否在珠子上形成SC5b-9或补体的膜攻击复合物（C5b-9）。
