Department of Urology, The Fourth Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China.
Department of Urology, The Affiliated Taizhou Second People's Hospital of Yangzhou University, Taizhou, Jiangsu, China.
Cell Cycle. 2023 Sep;22(18):2003-2017. doi: 10.1080/15384101.2023.2271341. Epub 2023 Nov 23.
Gastric cancer is a common malignant tumor of the digestive tract and the fourth leading cause of death from cancer-related diseases. In recent years, many studies have found that circular RNAs play an important role in cancer. Tumor-associated macrophages (TAMs) are also critical for tumor progression.
This study examined the role of circRNA_102191 in gastric cancer progression.
The relative mRNA levels were determined by qRT-PCR. Western blotting and ELISA were used to detect the protein levels. In vitro proliferation was assessed using CCK8 and clonogenic assays. The migration and invasion of cell lines were assessed by transwell-based assays. The interactions between molecules were detected using a luciferase reporter assay. M0 macrophages were induced with PMA. M1 macrophages were induced with LPS and IFN-γ, and M2 macrophages were induced with IL-4.
The expression of circRNA_102191 was enhanced significantly in gastric cancer cell lines and clinical tumor tissues. CircRNA_102191 promotes gastric cancer cell progression by regulating miR-493-3p and its downstream target gene XPR1. CircRNA_102191 can enhance the EMT process of gastric cancer cells by promoting the M2 polarization of macrophages.
CircRNA_102191 promotes the biological function of gastric cancer cells by regulating the miR-493-3p/XPR1 axis and M2 macrophage polarization.
胃癌是一种常见的消化道恶性肿瘤，也是癌症相关死亡的第四大主要原因。近年来，许多研究发现环状 RNA 在癌症中发挥重要作用。肿瘤相关巨噬细胞（TAMs）对于肿瘤的进展也至关重要。
本研究探讨了环状 RNA_102191 在胃癌进展中的作用。
通过 qRT-PCR 测定相对 mRNA 水平。使用 Western blot 和 ELISA 检测蛋白水平。通过 CCK8 和集落形成实验评估细胞系的体外增殖。通过基于 Transwell 的实验评估细胞系的迁移和侵袭。使用荧光素酶报告基因实验检测分子间的相互作用。用 PMA 诱导 M0 巨噬细胞。用 LPS 和 IFN-γ 诱导 M1 巨噬细胞，用 IL-4 诱导 M2 巨噬细胞。
环状 RNA_102191 在胃癌细胞系和临床肿瘤组织中表达显著增强。CircRNA_102191 通过调节 miR-493-3p 及其下游靶基因 XPR1 促进胃癌细胞的进展。CircRNA_102191 可以通过促进巨噬细胞的 M2 极化来增强胃癌细胞的 EMT 过程。
CircRNA_102191 通过调节 miR-493-3p/XPR1 轴和 M2 巨噬细胞极化来促进胃癌细胞的生物学功能。
