Décarie-Spain Léa, Gu Cindy, Lauer Logan Tierno, Subramanian Keshav S, Chehimi Samar N, Kao Alicia E, Deng Iris, Bashaw Alexander G, Klug Molly E, Galbokke Ashyah Hewage, Donohue Kristen N, Yang Mingxin, de Lartigue Guillaume, Myers Kevin P, Crist Richard C, Reiner Benjamin C, Hayes Matthew R, Kanoski Scott E
Human & Evolutionary Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, California, United States.
Neuroscience Graduate Program, University of Southern California, Los Angeles, California, United States.
bioRxiv. 2023 Oct 10:2023.10.10.561731. doi: 10.1101/2023.10.10.561731.
The ability to encode and retrieve meal-related information is critical to efficiently guide energy acquisition and consumption, yet the underlying neural processes remain elusive. Here we reveal that ventral hippocampus (HPCv) neuronal activity dynamically elevates during meal consumption and this response is highly predictive of subsequent performance in a foraging-related spatial memory task. Targeted recombination-mediated ablation of HPCv meal-responsive neurons impairs foraging-related spatial memory without influencing food motivation, anxiety-like behavior, or escape-mediated spatial memory. These HPCv meal-responsive neurons project to the lateral hypothalamic area (LHA) and single-nucleus RNA sequencing and in situ hybridization analyses indicate they are enriched in serotonin 2a receptors (5HT2aR). Either chemogenetic silencing of HPCv-to-LHA projections or intra-HPCv 5HT2aR antagonist yielded foraging-related spatial memory deficits, as well as alterations in caloric intake and the temporal sequence of spontaneous meal consumption. Collective results identify a population of HPCv neurons that dynamically respond to eating to encode meal-related memories.
编码和检索与进食相关信息的能力对于有效指导能量获取和消耗至关重要，但其潜在的神经过程仍然难以捉摸。在这里，我们揭示腹侧海马体（HPCv）神经元活动在进食过程中会动态升高，并且这种反应高度预测了随后在觅食相关空间记忆任务中的表现。靶向重组介导的HPCv进食反应神经元消融会损害觅食相关空间记忆，而不会影响食物动机、焦虑样行为或逃避介导的空间记忆。这些HPCv进食反应神经元投射到下丘脑外侧区（LHA），单核RNA测序和原位杂交分析表明它们富含5-羟色胺2a受体（5HT2aR）。HPCv到LHA投射的化学遗传学沉默或HPCv内5HT-2aR拮抗剂都会导致觅食相关空间记忆缺陷，以及热量摄入和自发进食时间序列的改变。综合结果确定了一群HPCv神经元，它们对进食做出动态反应以编码与进食相关的记忆。
