Choi Junsik, Gehring Mary
Whitehead Institute for Biomedical Research, Cambridge MA 02142.
Dept. of Biology, Massachusetts Institute of Technology, Cambridge MA 02139.
bioRxiv. 2023 Oct 3:2023.10.03.560721. doi: 10.1101/2023.10.03.560721.
The nuclear lamina, a sub-nuclear protein matrix, maintains nuclear structure and genome function. Here, we investigate the role of Arabidopsis lamin analogs CROWDED NUCLEIs during gametophyte and seed development. We observed defects in mutant seeds, including seed abortion and reduced germination rate. Quadruple null genotypes were rarely transmitted through gametophytes. We focused on the () endosperm, which exhibited enlarged chalazal cysts and increased expression of stress-related genes and the MADS-box transcription factor and its targets. Previously, it was shown that is regulated by H3K27me3 and that CRWN1 interacts with the PRC2 interactor PWO1. Thus, we tested whether alters H3K27me3 patterns. We observed a mild loss of H3K27me3 at several hundred loci, which differed between endosperm and leaves. These data indicate that CRWNs are necessary to maintain the H3K27me3 landscape, with tissue-specific chromatin and transcriptional consequences.
核纤层是一种核内亚蛋白基质，维持着核结构和基因组功能。在此，我们研究了拟南芥核纤层类似物CROWDED NUCLEIs在配子体和种子发育过程中的作用。我们在突变体种子中观察到了缺陷，包括种子败育和发芽率降低。四重无效基因型很少通过配子体传递。我们聚焦于（）胚乳，其表现出合点囊肿增大以及应激相关基因、MADS盒转录因子及其靶标的表达增加。此前研究表明，（此处原文缺失相关内容）受H3K27me3调控，且CRWN1与PRC2相互作用因子PWO1相互作用。因此，我们测试了（此处原文缺失相关内容）是否会改变H3K27me3模式。我们在数百个位点观察到H3K27me3轻度缺失，胚乳和叶片中的情况有所不同。这些数据表明，CRWNs对于维持H3K27me3格局是必要的，具有组织特异性的染色质和转录后果。
