Department of Orthopaedic Surgery, University of Southern California, Los Angeles, CA 90033, USA; Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Department of Orthopaedic Surgery, University of Southern California, Los Angeles, CA 90033, USA; Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles, CA 90033, USA.
STAR Protoc. 2023 Dec 15;4(4):102656. doi: 10.1016/j.xpro.2023.102656. Epub 2023 Oct 22.
Muscle stem cells (MuSCs) are the building blocks for regenerating skeletal muscle after trauma. If we intend to maximize the therapeutic potential of MuSCs, we must further study their molecular and functional properties. Here, we present a protocol for the isolation of mouse MuSCs via a two-step enzymatic and mechanical dissociation of skeletal muscle coupled with fluorescence-activated cell sorting (FACS). FACS-isolated MuSCs can be used for various downstream applications including cell culture, cell transduction, immunofluorescence, and gene expression assays. For complete details on the use and execution of this protocol, please refer to Almada et al. (2021)..
肌干细胞（MuSCs）是创伤后再生骨骼肌的构建块。如果我们打算最大限度地发挥 MuSCs 的治疗潜力，就必须进一步研究它们的分子和功能特性。在此，我们提供了一种通过两步酶解和机械解聚结合荧光激活细胞分选（FACS）分离小鼠 MuSCs 的方案。FACS 分离的 MuSCs 可用于各种下游应用，包括细胞培养、细胞转导、免疫荧光和基因表达分析。有关该方案使用和实施的完整详细信息，请参考 Almada 等人。（2021）。
