PPIH 基因调控系统及其在肝细胞癌中的预后意义：综合分析。

PPIH gene regulation system and its prognostic significance in hepatocellular carcinoma: a comprehensive analysis.

机构信息

Department of Clinical Laboratory, The Second Affiliated Hospital of Guizhou Medical University, Kaili, Guizhou 556000, China.

School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.

出版信息

Aging (Albany NY). 2023 Oct 23;15(20):11448-11470. doi: 10.18632/aging.205134.

DOI:10.18632/aging.205134

PMID:37874737

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10637785/

Abstract

BACKGROUND

Peptidyl-prolyl isomerase H (PPIH) is a member of the cyclophilin protein family, which functions as a molecular chaperone and is involved in the splicing of pre-mRNA. According to reports, the malignant progression of HCC related to hepatitis B virus (HBV) is tightly associated with RNA-binding proteins. Nevertheless, there is no research on PPIH expression or its function in the occurrence and progression of HCC.

RESULTS

We are the first to reveal that the mRNA and protein levels of are substantially overexpressed in HCC, as the outcomes show. A significant correlation existed between enriched expression of within HCC and more advanced, poorly differentiated, and TP53-mutated tumors.

CONCLUSION

These findings, which suggest that may serve as a predictive biomarker for people with HCC, serve as a starting point for further investigation into the function of in the progression of carcinogenesis.

METHODS

Accordingly, we utilized clinical samples and bioinformatics analysis to assess mRNA, protein expression, and gene regulatory system in HCC. Additionally, Wilcoxon signed-rank testing and logistic regression were utilized to inspect the association between clinicopathological factors and . Clinical pathological traits linked to overall survival (OS) among HCC patients were examined via TCGA data via Cox regression and the Kaplan-Meier approach. Additionally, via TCGA data collection, gene set enrichment assessment was also conducted.

摘要

背景

肽基脯氨酰顺反异构酶 H（PPIH）是亲环素蛋白家族的一员，作为分子伴侣发挥作用，并参与前体 mRNA 的剪接。据报道，乙型肝炎病毒（HBV）相关 HCC 的恶性进展与 RNA 结合蛋白密切相关。然而，目前尚无关于 PPIH 在 HCC 发生和进展中表达或功能的研究。

结果

我们首次揭示在 HCC 中，的 mRNA 和蛋白水平显著过表达。结果表明，在 HCC 中的富集表达与更晚期、低分化和 TP53 突变的肿瘤显著相关。

结论

这些发现表明，可能作为 HCC 患者的预测生物标志物，为进一步研究在致癌发生进展中的功能提供了起点。

方法

因此，我们利用临床样本和生物信息学分析来评估 HCC 中的 mRNA、蛋白表达和基因调控系统。此外，我们还利用 Wilcoxon 符号秩检验和逻辑回归来检测与临床病理因素之间的关联。通过 Cox 回归和 Kaplan-Meier 方法，利用 TCGA 数据检测 HCC 患者与总生存期（OS）相关的临床病理特征。此外，我们还通过 TCGA 数据收集进行了基因集富集评估。